-
Plasmid#196280PurposeCassette used in dual sgRNA library generationDepositorInserthU6-sgRNA-CR3
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKE011
Plasmid#207912PurposeLevel 0 for gRNA in C4 module (5' GCAG-3' TCAG)DepositorInsertpU3-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS018
Plasmid#207911PurposeLevel 0 for gRNA in C3 module (5' TTCG-3' GCAG)DepositorInsertpU6-AarI-scaffold-term
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-dErbB1
Plasmid#197354PurposeA knock-out vector for dog ErbB1.DepositorInsertA gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010-Target1 (Mpphot)
Plasmid#186728PurposeBinary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).DepositorInsertMphot
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN1
Plasmid#194439PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout CEN1DepositorInsertgRNA targeting CEN1
UseCRISPRTagsExpressionPlantMutationPromoterMtU6Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgPIGA guide 1
Plasmid#193610PurposePIGA knockoutDepositorInsertsgPIGA guide 1 (PIGA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgPIGW guide 1
Plasmid#193612PurposePIGW knockoutDepositorInsertsgPIGW guide 1 (PIGW Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgPIGW guide 2
Plasmid#193613PurposePIGW knockoutDepositorInsertsgPIGW guide 2 (PIGW Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgNFkBIA guide 1
Plasmid#193595PurposeNFkBIA knockoutDepositorInsertsgNFkBIA guide 1 (NFKBIA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgSNRPA guide 1
Plasmid#193598PurposeSNRPA knockoutDepositorInsertsgSNRPA guide 1 (SNRPA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgSNRPA guide 2
Plasmid#193599PurposeSNRPA knockoutDepositorInsertsgSNRPA guide 2 (SNRPA Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_ST-sgRNA
Plasmid#188705PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting lociDepositorInsertST sgRNAs
UseLentiviralTagsExpressionMammalianMutationPromoterhUbCAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC3
Plasmid#191858PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_DRB
Plasmid#164992PurposeExpression of gRNA targeting HLA-DRB locus, including DRB1*01:02:01DepositorInsertgRNA against HLA-DRB1*01:02:01
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_DQA
Plasmid#164990PurposeExpression of gRNA targeting HLA-DQA locus, including DQA1*01:01:01DepositorInsertgRNA against HLA-DQA1*01:01:01
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_HLA_DPA
Plasmid#164988PurposeExpression of gRNA targeting HLA-DPA locus, including DPA1*02:01:08DepositorInsertgRNA against HLA-DPA1*02:01:08
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_DHFR
Plasmid#183281PurposeAll-in-One CRISPRko system with a guide RNA that targets DHFR geneDepositorInsertDHFR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only