We narrowed to 13,296 results for: SHI

1. Mammalian_SaAID3S

   Plasmid

   #188651

   Purpose

   Expresses SaAID3S and gRNA in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Inserts

   nSaCas9-AID3S--UGI
   Sa-sgRNA
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SaCas9

   Promoter

   EF-1a and H1

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mammalian_SaAID2S

   Plasmid

   #188652

   Purpose

   Expresses SaAID2S and gRNA in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Inserts

   AID2S-nSaCas9--UGI
   Sa-sgRNA
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SaCas9

   Promoter

   EF-1a and H1

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLR5-CAG-NLS-tdiRFP670

   Plasmid

   #82621

   Purpose

   Expresses NLS-tdiRFP670 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   NLS-tdiRFP670
   

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBSKΔB-CBh-Cas9n-pA

   Plasmid

   #82617

   Purpose

   Expresses Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

   Insert

   SpCas9n
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_eGFP-pScaC-MTS

   Plasmid

   #228827

   Purpose

   For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cells

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   ScaC
   

   Use

   Tags

   MTS and eGFP

   Expression

   Mammalian

   Mutation

   aa 33-223

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIVEX2.3-AC17-5c-frGFP

   Plasmid

   #217525

   Purpose

   AC17-5c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

   Depositor

   Keisuke Fukunaga

   Article

   Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

   Insert

   AC17-5c riboswitch-regulated folding reporter GFP
   

   Use

   In vitro transcription-translation

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIVEX2.3-Theo-3c-EGFP

   Plasmid

   #217526

   Purpose

   Theo-3c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

   Depositor

   Keisuke Fukunaga

   Article

   Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

   Insert

   Theo-3c riboswitch-regulated EGFP
   

   Use

   In vitro transcription-translation

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRESneo-FLAG/HA-FL

   Plasmid

   #215839

   Purpose

   Expression of FLAG/HA tagged firefly luciferase (FL)

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

   Insert

   FLAG/HA tagged firefly luciferase (FL)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. psiCHECK1-CXCR4-MM4

   Plasmid

   #215846

   Purpose

   Expression of CXCR4 target with mismatch (MM) 4_

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

   Insert

   CXCR4 target with mismatch (MM) 4x
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. psiCHECK1-CXCR4

    Plasmid

    #215845

    Purpose

    Expression of CXCR4 target with perfect-match site

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

    Insert

    CXCR4 target with perfect-match site
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-FLAG/HA-p54

    Plasmid

    #215844

    Purpose

    Expression of FLAG/HA-p54

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

    Insert

    Human p54
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB602-po-mCerulean

    Plasmid

    #213861

    Purpose

    Binary vector for the expression of peroxisome transit peptide fused mCerulean in plants (for Agrobacterium-mediated genetic transformation and visualization of peroxisome.)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    Peroxisome transit peptide fused mCerulean
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Yeast_Target-AID2S

    Plasmid

    #188647

    Purpose

    Expressing Target-AID2S in yeasts

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID2S-UGI
    

    Use

    Tags

    UGI

    Expression

    Yeast

    Mutation

    D10A for SpCas9

    Promoter

    pGal1

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLR5-U6-sgRNA-EF

    Plasmid

    #82635

    Purpose

    piggyBac vector for expressing modified sgRNA in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Type

    Empty backbone

    Use

    CRISPR; Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pISH-Vmn2r76

    Plasmid

    #108084

    Purpose

    Preparation of RNA probe for ISH of mouse Vmn2r76 gene

    Depositor

    Peter Mombaerts

    Article

    Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

    Insert

    Vmn2r76 (Vmn2r76 Mouse)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmyc-GFP-TNRC6C-(928-1020)

    Plasmid

    #42026

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6C (TNRC6C Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa928-1020

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-EML4-ALK variant 3a I1171N/D1203N

    Plasmid

    #183833

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    I1171N/D1203N

    Promoter

    CMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. plk1 WT GFP-myc

    Plasmid

    #132963

    Purpose

    To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

    Depositor

    Michael Yaffe

    Article

    Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

    Insert

    polo-like kinase 1 (PLK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRXIP-3xMyc-STX17FL

    Plasmid

    #89942

    Purpose

    Expresses syntaxin17 tagged with Myc in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

    Insert

    human syntaxin17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSETB/mStayGold

    Plasmid

    #212017

    Purpose

    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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