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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 2221 - 2240 of 14582 results

  3. gRNA_DNMT3a-T2

    Plasmid
    #41822
    Purpose
    Expresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineering
    Depositor
    George Church
    Publication
    Mali et al Science. 2013 Feb 15;339(6121):823-6. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA_DNMT3a-T2 (DNMT3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Jan. 11, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Expression
    Bacterial and Mammalian
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113631-plasmid-map-sequence-id-222216

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  14. pEVOL-pAzF[TAG]

    Plasmid
    #164579
    Purpose
    Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons
    Depositor
    Ryan Mehl
    Publication
    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2
    Inserts
    Mj pCNFRS[TAG] (aaRS1)
    Mj pCNFRS[TAG] (aaRS2)
    Mj tRNA[TAG]
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    araBAD, glnS, and proK
    Available since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164579-plasmid-map-sequence-id-392918

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  16. pDN34

    Plasmid
    #61343
    Purpose
    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)
    Depositor
    Barbara Di Ventura, Roland Eils
    Publication
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Tags
    GS linker-AsLov2-biNLS2 and PKIt NES
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61343-plasmid-map-sequence-id-228919

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  17. pCool-mRbx1-His-hCul1-CTP

    Plasmid
    #29519
    Depositor
    Ning Zheng
    Insert
    Rbx1 CUL1 (CUL1, Rbx1 Human, Mouse)
    Tags
    GST, His (before thrombin), and Thrombin Cut Site…
    Expression
    Bacterial
    Available since
    May 26, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 2221 - 2240 of 14582 results