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pcDNA3-sACE2(WT)

Plasmid

#145147

Purpose

Mammalian expression plasmid for soluble ACE2 (protease domain)

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Splicing-targeting CRISPR-Cas9 library for human lncRNAs

Pooled library

#119977

Purpose

Designed for genomic deletion screening of functional lncRNAs by targeting their splice sites.

Depositor

Wensheng Wei

Publication

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only
mRNA-CleanCap entry vector

Plasmid

#188249

Purpose

Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

Depositor

Howard Chang

Publication

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pKM491-Flag-His

Plasmid

#109282

Purpose

ORBIT integrating plasmid for C-terminal tagging with FLAG-His tag

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Flag-His

Use

Orbit integrating plasmid

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hSyn1-tTA

Plasmid

#99119

Purpose

An AAV genome that expresses tTA from the hSyn1 promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

hSyn1

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAsCpf1(TATV)(BB) (pY221)

Plasmid

#89354

Purpose

Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

Depositor

Feng Zhang

Publication

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1 RVR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K548V/N552R

Promoter

CBh

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

Plasmid

#31477

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 T2A mTagBFP

Use

Lentiviral

Tags

HA and NLS

Mutation

F13Y + S111Y on I-AniI = Y2 "reo" (res…

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-tdTomato

Plasmid

#99116

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomato

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-tdTomato

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSP64TS (DM#117)

Plasmid

#15034

Depositor

Douglas Melton

Publication

Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

Insert

beta-globin 5' and 3' UTR

Expression

Bacterial

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pDIRECT_25F

Plasmid

#91143

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA

Use

CRISPR

Expression

Plant

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pSP64T (DM#111)

Plasmid

#15030

Depositor

Douglas Melton

Publication

Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

Insert

beta-globin 5' and 3' UTR

Expression

Bacterial

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCS6-YFP-SMASh(AI)

Plasmid

#111500

Purpose

Expresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].

Depositor

Michael Lin

Publication

Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

Insert

YFP-SMASh(AI)

Tags

HA epitope tag and SMASh tag

Expression

Mammalian

Mutation

The HCV NS3 protease carries V36M, T54A, and S122…

Promoter

CMV

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-PE2-NG

Plasmid

#159977

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Publication

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-NG

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBABE MDER

Plasmid

#13494

Depositor

Stephen Tapscott

Publication

Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

Insert

MyoD1-Estrogen Receptor Fusion Protein (Myod1 Human, Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

full length MyoD was fused to aa282-595 of Esr1. …

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCFJ1532 - pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

Plasmid

#159807

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-1 | mosase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagBFP-2A-BSD

Plasmid

#167929

Purpose

Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Publication

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Human Metabolic Gene CRISPRa sgRNA library

Pooled library

#187080

Purpose

Library targeting 2,989 human metabolic genes for activation of gene expression.

Depositor

Kivanc Birsoy

Publication

Unlu et al Mol Cell. 2022 Aug 4;82(15):2832-2843.e7

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only
pLX304 Flag-mCherry-eEF1A1_IRES-GFP

Plasmid

#198383

Purpose

eEF1A1 fluorescent reporter

Depositor

Jack Taunton

Publication

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

eEF1A1 (EEF1A1 Human)

Use

Lentiviral

Tags

Flag-mCherry

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEERM3 Km

Plasmid

#64025

Purpose

Plasmid for integration into neutral site of Synechocystis 6803

Depositor

Pia Lindberg

Publication

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pFH2.2_mCherry-MS2x12

Plasmid

#205521

Purpose

Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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