We narrowed to 10,627 results for: otos
-
Plasmid#90214PurposeTo convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.DepositorAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only
-
AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP
Plasmid#132393PurposeExchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 siteDepositorInserthuman TauP301L 2N4R-EGFP (MAPT Human, Synthetic)
UseCRISPR and Cre/LoxTagsEGFPExpressionMammalianMutationProline 301 to Leucine (P301L)PromoterTREtightAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 CAG rtTA3 TauWT 2N4R-EGFP
Plasmid#132389PurposeExchange cassette containing inducible TauWT 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 siteDepositorInserthuman TauWT 2N4R-EGFP (MAPT Human, Synthetic)
UseCRISPR and Cre/LoxTagsEGFPExpressionMammalianPromoterTREtightAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-mXFPm-IFNGR1(18-489)
Plasmid#192786PurposeExpression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNGR1 (IFNGR1 Human)
TagsIg k-chain leader sequence and mXFPmExpressionMammalianMutationmXFPm: tryptophan 66 to phenylalanine, gluatmic a…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20_mStrawberry_Atg4BC74A
Plasmid#161735PurposeDoxycycline-inducible expression of Atg4B(C74A) fused with mStrawberry to inhibit autophagyDepositorInsertAtg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
UseLentiviral; Destinatioin vector for gateway cloni…TagsmStrawberryExpressionMammalianMutation220-222 TGC (Cys) is changed to GCA (Ala)Available SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-mXFPe-IFNGR2(30-337)
Plasmid#192787PurposeExpression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNGR2 (IFNGR2 Human)
TagsIg k-chain leader sequence and mXFPeExpressionMammalianMutationmXFPe: tyrosine 66 to phenylalanine, asparagine 1…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)
Plasmid#187844PurposeExpression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
TagsIg k-chain leader sequence, SNAPf-tag, and meGFPe…ExpressionMammalianMutationmeGFPe: asparagine 198 to aspartic acid and tyros…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-mCherry-PA-Rac1
Plasmid#169042PurposeMiddle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insertDepositorInsertPA-Rac-1 (RAC1 Human, Avena sativa (oat))
UseGateway entry cloning vectorTags6X His and Fused with mCherryMutationRac1 starts at I4 and contains mutations Q61L, E9…Available SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-LNI (with mNgn2x3HA)
Plasmid#233195PurposeRetroviral expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)
Plasmid#192785PurposeExpression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
TagsIg k-chain leader sequence, SNAPf-tag, and mXFPeExpressionMammalianMutationmXFPe: tyrosine 66 to phenylalanine, asparagine 1…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsynapsin-DIO-adra2a-shRNA-mCherry
Plasmid#245063PurposeAAV-transgene knocking down adra2a receptor (adrenergic 2a receptor) transcripts cell type-selectively. Using the pPRIME. system, this generates mir30-derived shRNAs and a marker from the same RNA.DepositorInsertAAV-human synapsin promoter-DIO-adra2a-shRNA-mCherry (Adra2a Synthetic, Mouse)
UseAAV, Cre/Lox, and RNAiPromoterhuman synapsinAvailable SinceOct. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-LNI-bGH
Plasmid#233203PurposeAAV expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogrammingDepositorAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-NIL (with mNgn2x3HA)
Plasmid#233192PurposeRetroviral expression of Ngn2x3HA, Isl1, and Lhx3 for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-NLI (with mNgn2x3HA)
Plasmid#233193PurposeRetroviral expression of Ngn2x3HA, Lhx3, and Isl1 for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-LIN (with mNgn2x3HA)
Plasmid#233194PurposeRetroviral expression of Lhx3, Isl1, and Ngn2x3HA for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ILN (with mNgn2x3HA)
Plasmid#233196PurposeRetroviral expression of Isl1, Lhx3, and Ngn2x3HA for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-INL (with mNgn2x3HA)
Plasmid#233197PurposeRetroviral expression of Isl1, Ngn2x3HA, and Lhx3 for motor neuron reprogrammingDepositorUseRetroviralTagsx3HAExpressionMammalianAvailable SinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)
Plasmid#192704PurposeExpression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
TagsIg k-chain leader sequence, SNAPf-tag, and meGFPm…ExpressionMammalianMutationmeGFPm: gluatmic acid 142 to asparagine and histi…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)
Plasmid#192784PurposeExpression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
TagsIg k-chain leader sequence, SNAPf-tag, and mXFPmExpressionMammalianMutationmXFPm: tryptophan 66 to phenylalanine, gluatmic a…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
p11-3’_8xN_PAM-site1 (RTW554)
Pooled Library#160132PurposePlasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)DepositorSpeciesSyntheticUseCRISPRAvailable SinceFeb. 8, 2021AvailabilityAcademic Institutions and Nonprofits only