We narrowed to 8,660 results for: pcDNA3

1. pCDNA3.1-hMcl-1(T163A)

   Plasmid

   #25376

   Depositor

   Roger Davis

   Article

   Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

   Insert

   hMCL1 (MCL1 Human)
   

   Tags

   V5 and His tag

   Expression

   Mammalian

   Mutation

   changed threonine 163 to alanine

   Available Since

   Aug. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1_mGFP-FGFR3-mScarlet

   Plasmid

   #192509

   Purpose

   Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc-mScarlet
   

   Tags

   mGFP and mScarlet

   Expression

   Mammalian

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Mcm2-mVen

   Plasmid

   #211398

   Purpose

   mammalian expression of human Mcm2 C-terminally fused to mVenus

   Depositor

   Helmut Pospiech

   Insert

   Mcm2 (MCM2 Human)
   

   Tags

   mVenus

   Expression

   Mammalian

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. N-TAP A2A in pcDNA3

   Plasmid

   #67850

   Purpose

   mammalian expression of A2A receptor with N terminal TAP tag

   Depositor

   Christian Gruber

   Article

   Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

   Insert

   adenosine A2a receptor (ADORA2A Human)
   

   Tags

   TAP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1/GS-DJ1-L166P-V5

   Plasmid

   #29343

   Depositor

   Mark Cookson

   Article

   Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

   Insert

   DJ1 (PARK7 Human)
   

   Tags

   His6 and V5 epitope

   Expression

   Mammalian

   Mutation

   L166P

   Available Since

   March 7, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pY015 (pcDNA3.1-hLiCpf1)

   Plasmid

   #69987

   Purpose

   Expresses humanized LiCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLiCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1(+)_Paragranulin-no linker

   Plasmid

   #176917

   Purpose

   express Paragranulin without linker 1 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Paragranulin (GRN Human)
   

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Cerulean-WW-NES

   Plasmid

   #84629

   Purpose

   Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   Cerulean-WW-NES
   

   Tags

   6xHis, Cerulean, Nuclear export signal (NES), T7 …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.3-hALDOA D34S

   Plasmid

   #210810

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3 14 mut B-Myb

    Plasmid

    #25967

    Depositor

    Rob Lewis

    Article

    Johnson et al J Biol Chem. 2002 Feb 8. 277(6):4088-97.

    Insert

    B-Myb (Mybl2 Mouse)
    

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Changed threonines 267, 408, 443, 447 490, 497, 5…

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1: TfR1-CoilE

    Plasmid

    #181988

    Purpose

    CoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    TfR1-CoilE
    

    Tags

    CoilE

    Expression

    Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Beta2-PAmcherry1

    Plasmid

    #114190

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Tags

    3HA, FLAG, and PAmcherry1

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Lyn-GR-AKAR3

    Plasmid

    #173016

    Purpose

    Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Lyn-GR-AKAR3
    

    Tags

    EGFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1/HisC 14-3-3 sigma

    Plasmid

    #11947

    Depositor

    Michael Yaffe

    Article

    Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

    Insert

    Stratifin (SFN Human)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-deltaA1-VWF

    Plasmid

    #124823

    Purpose

    Expresses multimeric human VWF protein without A1 domain. This construct has no tag.

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF without A1 domain (VWF Human)
    

    Expression

    Mammalian

    Mutation

    Deleted A1 domain

    Promoter

    CMV

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Y921F EphA2 pcDNA3

    Plasmid

    #102736

    Purpose

    Expression of EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

    Insert

    EphA2 (Epha2 Mouse)
    

    Expression

    Mammalian

    Mutation

    Y921F (TAC > TTC)

    Promoter

    CMV

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 merlin 1-332

    Plasmid

    #11625

    Depositor

    Vijaya Ramesh

    Article

    Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

    Insert

    merlin (NF2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Amino-terminal half of merlin.

    Available Since

    May 3, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-tRSA-G quartet

    Plasmid

    #32203

    Depositor

    Ian Macara

    Article

    Iioka et al Nucleic Acids Res. 2011 Apr;39(8):e53. E

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FLAG_FBXL7 (R310H)

    Plasmid

    #171849

    Purpose

    pcDNA3-FLAG_FBXL7 (R310H)

    Depositor

    Michele Pagano

    Article

    Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

    Insert

    F-box and leucine rich repeat protein 7 (FBXL7 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Arginine 310 to Histidine

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-hMcl-1(S159A)

    Plasmid

    #25374

    Depositor

    Roger Davis

    Article

    Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

    Insert

    hMCL1(S159A) (MCL1 Human)
    

    Tags

    V5 and His tag

    Expression

    Mammalian

    Mutation

    changed serine 159 to alanine

    Available Since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only