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TRE-mCherry-p2A-eNpHR3.0-TS

Plasmid

#158703

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

mCherry-p2A-eNpHR3.0-TS

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiLox7.0-mVenus-CAGEn (Nrdj1)

Plasmid

#241404

Purpose

Proximity-triggered Protein Trans-Splicing between mVenus and RFP, Figure 1 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

mVenus-CAGEn (Nrdj1)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiLox7.0-CAGEc-RFP (Nrdj1)

Plasmid

#241402

Purpose

Proximity-triggered Protein Trans-Splicing between mVenus and RFP, Figure 1 in Nature Chemical Biology 20.10 (2024): 1353-1360.

Depositor

Thomas Muir

Article

Lee et al Nat Chem Biol. 2024 Oct;20(10):1353-1360

Insert

CAGEc-RFP (Nrdj1)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_NANOS3_cterm

Plasmid

#222904

Purpose

Cas9/sgRNA plasmid for targeting NANOS3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NANOS3 sgRNA (NANOS3 Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pOSIP-KO (KanR, 186)

Plasmid

#45985

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8m

Plasmid

#169257

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B15

Plasmid

#135513

Purpose

Mammalian expression of myc-tagged HLA-B*15:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*15:01 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA PSPC1

Plasmid

#101764

Purpose

Expresses PSPC1 in mammalian cells

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

paraspeckle component 1 (PSPC1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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hNTo2-qgRNA-pYJA5

Plasmid

#217782

Purpose

Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

Depositor

Adriano Aguzzi

Article

Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

Insert

Quadruple sgRNA-expression cassette for nontargeting control for gene ablation

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

human U6, mouse U6, human H1, human 7SK

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMK293 (mAID-mCherry2-Hygro)

Plasmid

#72831

Purpose

C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mCherry2-Hygro

Use

CRISPR

Tags

mAID-mCherry2

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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