We narrowed to 2,327 results for: PUC19

1. pTR-459 tNGFR-P2A-STAT6

   Plasmid

   #186070

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   STAT6 (STAT6 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTR-460 tNGFR-P2A-STAT4

   Plasmid

   #186075

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   STAT4 (STAT4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTR-458 tNGFR-P2A-STAT2

   Plasmid

   #186076

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   STAT2 (STAT2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTR-455 tNGFR-P2A-WAS

   Plasmid

   #186082

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   WAS (WAS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLX-N-CTCF-mAID-Halo-donor

   Plasmid

   #139747

   Purpose

   Mouse N-terminal CTCF CRISPR knock-in donor vector with miniAID degron and HaloTag

   Depositor

   Robert Tjian

   Article

   Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

   Insert

   CTCF CRISPR knock-in (Ctcf Mouse)
   

   Use

   CRISPR

   Tags

   mAID-HaloTag

   Expression

   Mutation

   Promoter

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTR-469 JUNB-tNGFR

   Plasmid

   #186060

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   JUNB (JUNB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTR-472 FOXO1-tNGFR

   Plasmid

   #186062

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   FOXO1 (FOXO1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with tNGFR sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIG-721 CTLA4-GFP T124P

   Plasmid

   #186126

   Purpose

   CTLA4 gene knockin with mutation

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CTLA4 (CTLA4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   T124P

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIG-648 CTLA4int1-CTLA4-GFP-SV40

   Plasmid

   #186123

   Purpose

   CTLA4 gene knockin

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   CTLA4 (CTLA4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   WT with GFP sequence

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTR-374 tNGFR-P2A-B2M

    Plasmid

    #186068

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    B2M (B2M Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTR-471 IRF4-tNGFR

    Plasmid

    #186061

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IRF4 (IRF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. P521_VSX2_p2A-h2b_mRuby3

    Plasmid

    #239104

    Purpose

    Used in CRISPR gene editing to Insert a p2A-h2b_mRuby3 sequence before the stop codon of the endogenous human VSX2 gene

    Depositor

    Karl Wahlin

    Article

    Jones et al Cells. 2022 Oct 28;11(21):3412. doi: 10.

    Insert

    VSX2 (VSX2 Human)
    

    Use

    CRISPR

    Tags

    -p2A-h2b-mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous VSX2 promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. P530_SIX6-p2A-h2b-EGFP

    Plasmid

    #239101

    Purpose

    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of SIX6. This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

    Depositor

    Karl Wahlin

    Article

    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

    Insert

    SIX6 (SIX6 Human)
    

    Use

    Donor plasmid

    Tags

    p2A-h2b-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous SIX6 promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMT1219

    Plasmid

    #228301

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Synthetic, Severe acute respiratory syndrome-related coronavirus 2)
    

    Use

    Synthetic Biology

    Tags

    GGG linker followed by His6 and MF_ secretion sig…

    Expression

    Yeast

    Mutation

    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIG-749_HA-GD2-28z_CAR_TFAP4

    Plasmid

    #207490

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

    Plasmid

    #207493

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIG-851_HA-GD2-28z_HA-TFAP4

    Plasmid

    #207501

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIG-852_HA-GD2-28z_TFAP4-HA

    Plasmid

    #207502

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4-HA, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIG-719 CTLA4-GFP R70W

    Plasmid

    #186124

    Purpose

    CTLA4 gene knockin with mutation

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    R70W

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIG-720 CTLA4-GFP R75W

    Plasmid

    #186125

    Purpose

    CTLA4 gene knockin with mutation

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    R75W

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge