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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 60,538 results for: Gene

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Showing: 3361 - 3390 of 60538 results

  2. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid
    #98219
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Aurora_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…
    Promoter
    human synapsin
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98219-plasmid-map-sequence-id-256052

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  6. pUC19-Q927P4

    Plasmid
    #103131
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103131-plasmid-map-sequence-id-201895

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  7. pUC19-C7TEQ6

    Plasmid
    #103092
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    C7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103092-plasmid-map-sequence-id-201803

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  8. pUC19-A0LWB3

    Plasmid
    #103070
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    A0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103070-plasmid-map-sequence-id-201692

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  13. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

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  18. pRvCAHS1-mEGFP-NLS

    Plasmid
    #205013
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
    Use
    Tardigrade expression
    Available Since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205013-plasmid-map-sequence-id-405257

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  19. pRvSAHS1-mCherry-NLS

    Plasmid
    #205009
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
    Use
    Tardigrade expression
    Available Since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205009-plasmid-map-sequence-id-405252

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  24. pAAV-Ef1a-DIO ChETA-EYFP (AAV5)

    Viral Prep
    #26968-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO ChETA-EYFP (#26968). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO ChETA-EYFP plasmid DNA. EF1a-driven, Cre-dependent, ChETA fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available Since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-hSyn-GRAB_NE1h (AAV9)

    Viral Prep
    #123309-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_NE1h (#123309). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_NE1h plasmid DNA. hSyn-driven expression of GRAB-NE1h norepinephrine sensor in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Promoter
    Syn
    Available Since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only

  28. pAAV-CAG-dLight1.3b (AAV9)

    Viral Prep
    #125560-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci
    Promoter
    CAG
    Available Since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  29. pAAV.hSyn-FLEX.iGABASnFR (AAV1)

    Viral Prep
    #112163-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn-FLEX.iGABASnFR (#112163). In addition to the viral particles, you will also receive purified pAAV.hSyn-FLEX.iGABASnFR plasmid DNA. Synapsin-driven, Cre-dependent iGABASnFR GABA sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi
    Promoter
    Syn
    Available Since
    Jan. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 3361 - 3390 of 60538 results