We narrowed to 15,989 results for: LEA

1. Hy_pMT Flag-URH49 MUT

   Plasmid

   #110126

   Purpose

   Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   URH49 (DDX39A Human)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 amino acid mutations (RSFS motif changed to KSL…

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hy_pMT Flag-Hel25E MUT

   Plasmid

   #110122

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 mutations (KKLN motif changed to RSFS)

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. NKP91 GFP (1034)

   Plasmid

   #62023

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   Tmem41A (TMEM41A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

   Plasmid

   #134867

   Purpose

   Expresses EGFP-tagged nuclear envelope marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lamin A (LMNA Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

   Plasmid

   #132771

   Purpose

   Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

   Depositor

   Jan Lammerding

   Article

   Denais et al Science. 2016 Apr 15;352(6283):353-8. do

   Insert

   Cyclic GMP-AMP synthase (CGAS Human)
   

   Use

   Lentiviral

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   E225A D227A

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSN-A112C-GpA-G83I

   Plasmid

   #191239

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
   

   Tags

   nuclease A from S. aureus fused to the TM domain …

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine in SN, and Glycin…

   Promoter

   T7 promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNA 3C myc scfvFLAG

   Plasmid

   #234990

   Purpose

   Expression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG protein

   Depositor

   Daniel Leahy, Monica Gonzalez-Magaldi

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   NA-3C-myc-scfvFLAG
   

   Expression

   Mammalian

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIG-hNFATc2

   Plasmid

   #74050

   Purpose

   retroviral expression plasmid for human NFATc2/C

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human NFATc2, isoform C (NFATC2 Human)
   

   Use

   Retroviral

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   silent mutation A1723C in the sequence of NM_1730…

   Promoter

   pMSCV-LTRs

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. FASTHDR-tRNApromo-sgRNA-espCas91_1

   Plasmid

   #167210

   Purpose

   Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid

    #167288

    Purpose

    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    DDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    APEX2 and EGFP

    Expression

    Mammalian

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-NLS-actin

    Plasmid

    #118380

    Purpose

    Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Mutation

    NLS

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. COVID-SARS2 NSP13

    Plasmid

    #159614

    Purpose

    Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

    Depositor

    Opher Gileadi

    Article

    Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

    Insert

    Covid-SARS2 Nsp13
    

    Tags

    His6-Zb-TEV

    Expression

    Bacterial

    Mutation

    Codon-optimized for E. coli expression

    Promoter

    T7 - LacO

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGCaMP6m-N3-TPC2

    Plasmid

    #80147

    Purpose

    GCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real time

    Depositor

    Santiago Di Pietro

    Article

    Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7

    Insert

    TPCN2 (TPCN2 Human)
    

    Tags

    GCaMP6m

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. METTL7A pEGFP-N2 (890)

    Plasmid

    #62017

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    METTL7A (METTL7A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pStreptagII 3C SMO

    Plasmid

    #234995

    Purpose

    Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    SMO (SMO Human)
    

    Tags

    Streptag-II, HRV 3C site

    Expression

    Mammalian

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEABR NA 3C FLAG SA

    Plasmid

    #234987

    Purpose

    For production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    EABR-NA
    

    Tags

    HRV 3C site, FLAG, Strep-Tactin (SA)

    Expression

    Mammalian

    Mutation

    In the Streptavidin coding sequence Glu44Val/Ser4…

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_HyPer7RgDAAO

    Plasmid

    #217746

    Purpose

    Donor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    HyPer7 D amino acid oxidase
    

    Tags

    Nuclear export signal

    Expression

    Mammalian

    Mutation

    Fused DAAO to the C-terminus of HyPer7 using a Gl…

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. NKP66 pEGFP-N2 (1176)

    Plasmid

    #62039

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

    Insert

    MARCHV (MARCHF5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. CRF1R-GFP

    Plasmid

    #208912

    Purpose

    encodes the human corticotropin releasing factor 1 receptors, tagged with YFP

    Depositor

    Christian Gruber

    Article

    Fahradpour et al Front Pharmacol. 2017 Sep 25;8:616. doi:

    Insert

    CRHR1 (CRHR1 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

    Plasmid

    #182395

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-VenusN154
    VNp-LZ-VenusC155
    

    Tags

    Vesicle Nucleating peptide (VNp) & Leucine Zi…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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