We narrowed to 5,627 results for: mcs
-
Plasmid#194362PurposeSplit TEV assaysDepositorAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only
-
pODN-H2BC11-TagBFP
Plasmid#183867PurposeRepair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.DepositorInsertH2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
UseCRISPR; Donor templateTagslinker-mTagBFPExpressionMammalianMutationHomology arms contain point mutations to remove t…Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-ECT2_sgRNA
Plasmid#183875PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.DepositorAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-43
Plasmid#172759PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-43
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA3
Plasmid#171001PurposeHiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[Y536delinsFIV]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-35
Plasmid#172755PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-35
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-19
Plasmid#172748PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-19
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-3
Plasmid#172740PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-3
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-12
Plasmid#172744PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-12
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-14
Plasmid#172745PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-14
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-neoR
Plasmid#117048PurposeIn vitro expression of neoR (kanamycin resistance gene) from the T7 promoterDepositorInsertneoR
ExpressionBacterialPromoterT7Available SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a D540K
Plasmid#53057PurposeExpresses alpha subunit of D540K hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionMutationchanged Aspartate 540 to LysinePromoterSP6Available SinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a S620T
Plasmid#53059PurposeExpresses alpha subunit of S620T hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionMutationchanged Serine 620 to ThreoninePromoterSP6Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a S631A
Plasmid#53058PurposeExpresses alpha subunit of S631A hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionMutationchanged Serine 631 to AlaninePromoterSP6Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDLucBiDi
Plasmid#219562PurposeDual luciferase plasmid with Renilla luciferase constitutively expressed and firefly luciferase downstream of MCS without a start codonDepositorTypeEmpty backboneUseLuciferaseExpressionMammalianPromoterSV40 and T7Available SinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2-nbt300 2n-KalNFB-3xmir1T-syt2a
Plasmid#196900Purposezebrafish expressionDepositorInsertnbt300 2n-KalNFB-3xmir1T-syt2a
UseZebrafish expressionAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM-PAT-ProATE2 GW
Plasmid#80682Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneExpressionPlantPromoterProATE2Available SinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAM-PAT-ProATE1 GW
Plasmid#80681Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneExpressionPlantPromoterProATE1Available SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
MCJ.559
Bacterial Strain#69493PurposeStrain with recoded TAG and genomic deletions for improved incorporationDepositorBacterial ResistanceAmpicillinSpeciesEschericia coliAvailable SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-copGFP
Plasmid#73030PurposeExpress copGFPDepositorInsertcopGFP
UseLentiviralExpressionMammalianPromoterEF1Available SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only