We narrowed to 9,217 results for: sgRNA

Showing: 2241 - 2260 of 9217 results

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pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA

Plasmid

#86986

Purpose

For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein

Depositor

Ralf Kuehn

Publication

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

Cas9-venus

Tags

Cas9-Venus fusion protein

Expression

Mammalian

Promoter

CAG

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles
ULK1 sgRNA

Plasmid

#207559

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

Depositor

Jens Schmidt

Publication

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CCAGCCAGGCCAGAAAGGTC

Expression

Mammalian

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ATG5 sgRNA

Plasmid

#207555

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ATG5 locus

Depositor

Jens Schmidt

Publication

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AACTTGTTTCACGCTATATC

Expression

Mammalian

Promoter

CMV and U6

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgABCC4_1

Plasmid

#217433

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Publication

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 1 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgABCC4_3

Plasmid

#217434

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Publication

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 3 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_DAZL_cterm2

Plasmid

#222919

Purpose

Cas9/sgRNA plasmid for targeting DAZL

Depositor

George Church

Publication

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, DAZL sgRNA 2 (DAZL Human, Synthetic)

Use

CRISPR

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_DAZL_cterm3

Plasmid

#222920

Purpose

Cas9/sgRNA plasmid for targeting DAZL

Depositor

George Church

Publication

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, DAZL sgRNA 3 (DAZL Human, Synthetic)

Use

CRISPR

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

Plasmid

#218666

Purpose

sgRNA targeting human EIF2AK3/PERK

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK3 (EIF2AK3 Human)

Use

CRISPR and Lentiviral

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9_U6_sgRNA_Mettl3C

Plasmid

#165420

Purpose

sgRNA construct for targeting Mettl3 C-terminus

Depositor

Neil Brockdorff

Publication

Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

Insert

PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)

Use

CRISPR

Expression

Mammalian

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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BPK2301

Plasmid

#65778

Purpose

Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

Depositor

Keith Joung

Publication

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

St1Cas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJZC101

Plasmid

#62335

Purpose

sgRNA (no RNA aptamer addition) with COM-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
COM-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP gRNA (BRDN0000561167)

Plasmid

#80034

Purpose

3rd generation lentiviral gRNA plasmid targeting EGFP

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pME-Cas9

Plasmid

#64237

Purpose

expresses codon optimized Cas9 in zebrafish

Depositor

Wenbiao Chen

Publication

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

codon optimized Cas9

Use

CRISPR

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pX459-HypaCas9-RHOA_sgRNA

Plasmid

#183878

Purpose

pX459V2.0-HypaCas9 plasmid with RHOA sgRNA for N-terminal tagging of RhoA in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RHOA sgRNA spacer (RHOA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_1

Plasmid

#73533

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Publication

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_2

Plasmid

#73534

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Publication

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Ago2_1

Plasmid

#73529

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Publication

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pX330-sgRNA_Ago2_2

Plasmid

#73530

Purpose

Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Publication

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pT7-gfap-sgRNA

Plasmid

#65566

Purpose

in vitro trancription of sgRNA targeting the zebrafish gfap locus

Depositor

Jiu-lin Du

Publication

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap sgRNA (gfap Zebrafish)

Use

In vitro rna transcription

Promoter

T7

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHiFi Cas9-2×sgRNA (empty, donor)

Plasmid

#162277

Purpose

All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

Depositor

Kazuhisa Nakayama

Publication

Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HiFi Cas9

Use

CRISPR

Tags

BPNLS and FLAG tag

Expression

Mammalian

Mutation

SpCas9 (R691A)

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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