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pGP-AAV-syn-jGCaMP8m-WPRE

Plasmid

#162375

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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deltaNp63alpha-FLAG

Plasmid

#26979

Depositor

David Sidransky

Article

Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

Insert

ΔNP63alpha (TP63 Human)

Tags

FLAG

Expression

Mammalian

Mutation

dominant negative isoform of p63; aa 1–587

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pFB2G

Plasmid

#195290

Purpose

Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mTagBFP

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-Tol2 transposase

Plasmid

#158774

Purpose

Expression of Tol2 transposase

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

Tol2 tranposase

Expression

Mammalian

Mutation

Tol2 codon usage frequency well suitable for muri…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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EphA2-YFP

Plasmid

#108852

Purpose

Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

Depositor

Kalina Hristova

Article

Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

Insert

EPHA2 (EPHA2 Human)

Tags

Labeled with eYFP on the C-Terminus via a 15 amin…

Expression

Mammalian

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ153

Plasmid

#69304

Purpose

Gateway entry vector with pco-dCas9-3X(SRDX)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pco-dCas9-3X(SRDX) (Plant codon-optimized)

Use

CRISPR

Tags

2XFLAG

Mutation

SRDX (X3) repressor fusion; NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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cyto-Ruby3-iATPSnFR1.0

Plasmid

#102551

Purpose

expresses cytoplasmic ATP sensor in mammalian cells with red reference protein

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

Ruby3-iATPSnFR1.0

Tags

Ruby3

Expression

Mammalian

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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XLone-PE2

Plasmid

#136463

Purpose

Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment

Depositor

Xiaojun Lian

Insert

PE2

Expression

Mammalian

Promoter

TRE3GS

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pZR069_CROPseq-sgPTPRC-CRISPRi

Plasmid

#180271

Purpose

Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

PTPRC (CD45) sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR

Plasmid

#64927

Purpose

FRET-based biosensor for monitoring mTORC1 kinase activity.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

TORCAR (EIF4EBP1 Synthetic, Human)

Tags

Cerulean and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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