168,004 results

1. EF1a_FOXA1_P2A_Hygro_Barcode

   Plasmid

   #120438

   Purpose

   Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   FOXA1 (FOXA1 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.CamKII(1.3).eYFP.WPRE.hGH (AAV1)

   Viral Prep

   #105622-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.CamKII(1.3).eYFP.WPRE.hGH (#105622). In addition to the viral particles, you will also receive purified pAAV.CamKII(1.3).eYFP.WPRE.hGH plasmid DNA. Expression of EYFP from CaMKII(1.3) promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   CaMKII(1.3)

   Tags

   EYFP

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pUCmini-iCAP-AAV.CAP-B10

   Plasmid

   #175004

   Purpose

   non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

   Depositor

   Viviana Gradinaru

   Article

   Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

   Insert

   Synthetic construct isolate AAV.CAP-B10 VP1 gene
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   7 amino acid substitution between VP1 452 and VP1…

   Promoter

   p41

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF3-ultraID

   Plasmid

   #172878

   Purpose

   expresses ultraID in mammalian cells with the tet-on or tet-off system

   Depositor

   Julien Béthune

   Article

   Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

   Insert

   ultraID
   

   Use

   Luciferase and Retroviral; To be used in a tet-on…

   Tags

   myc

   Expression

   Mammalian

   Mutation

   fragment ([aa2-171]) of aquifex aeolicus BirA wit…

   Promoter

   TRE-cmv

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. BirA in pET28a (w400-2)

   Plasmid

   #26624

   Depositor

   Eric Campeau

   Insert

   BirA
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA Flag Lamp2b-HA

   Plasmid

   #71292

   Purpose

   Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Tags

   Flag, HA, and Lamp2 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)

   Viral Prep

   #137141-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   EYFP (Flp-dependent)

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pEGFP-FRB

   Plasmid

   #25919

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

   Insert

   FRB (MTOR Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV PHP.eB)

   Viral Prep

   #44361-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with the PHP.eB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Promoter

   Syn

   Tags

   mCherry (Cre-dependent)

   Available Since

   March 22, 2018

   Availability

   Academic Institutions and Nonprofits only

10. Anti-GFP [N86/38.1R]

    Plasmid

    #114492

    Purpose

    Mammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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