We narrowed to 14,071 results for: cas9

1. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

   Plasmid

   #194284

   Purpose

   EF1a-dCas9-KRAB-GFP with nontarget sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Promoter

   EF1a

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHDE-35S-Cas9-mCherry-UBQ

   Plasmid

   #78932

   Purpose

   For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs

   Depositor

   Yunde Zhao

   Article

   Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJEP313-pAAV-CMV-SaCas9-DIO-pA

   Plasmid

   #113690

   Purpose

   A CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9 (NEWENTRY )
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   NLS

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcs2+spCas9 ABE-Umax-nanos

   Plasmid

   #222142

   Purpose

   Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9 with nanos UTR
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Promoter

   SP6

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18T-mini-Tn7T-hph-dCas9

   Plasmid

   #218371

   Purpose

   Expression of dCas9 for CRISPR-interference

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PtetA

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKRG3-mU6-PUb-hSpCas9-pAc

   Plasmid

   #162163

   Purpose

   Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   T2A-pAc

   Expression

   Insect

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

   Plasmid

   #113163

   Purpose

   A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Px330-EGFP-LRRK2-CRISPR/Cas9

   Plasmid

   #180437

   Purpose

   Plasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   gRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

   Plasmid

   #98971

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNA and spCas9
   

   Expression

   Mammalian

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2T-CMV-evoCjCas9-ABE8e-BlastR

    Plasmid

    #194060

    Purpose

    Mammalian expression of evoCjCas9 ABE8e

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    evoCjCas9-ABE8e
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. peSpCas9(1.1)-2×sgRNA (empty, donor)

    Plasmid

    #80768

    Purpose

    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2T-cmv-SpCas9-TadCBEd-BlastR

    Plasmid

    #193848

    Purpose

    Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEd
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EFS-CjCas9-eGFP-HIF1a

    Plasmid

    #137929

    Purpose

    Expression of cjCas9 with Hif1a targeting sgRNA

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    SV40NLS-HA-T2A-EGFP

    Promoter

    EF-1alpha

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PET-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207374

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. NLS-dCas9-HA-NLS--GFP-p300

    Plasmid

    #183925

    Purpose

    p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    dCas9-GFP-p300
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pattB-LSL-AAVR-F2A-spCas9

    Plasmid

    #202459

    Purpose

    The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.

    Depositor

    Jan Carette

    Article

    Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d

    Insert

    AAVR (AU040320 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    mCherry fusion

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CASI-Cas9C-P2A-turboGFP

    Plasmid

    #80942

    Purpose

    Expresses Cas9C in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSHS207 - Bacterial expression plasmid for WT SpCas9

    Plasmid

    #101199

    Purpose

    Bacterial expression plasmid for WT SpCas9

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-FLEX-SaCas9-U6-sgDrd1

    Plasmid

    #159917

    Purpose

    Mutagenesis of Drd1

    Depositor

    Larry Zweifel

    Insert

    Drd1 (Drd1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. GCN5 core-dCas9-p300 core

    Plasmid

    #179553

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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