We narrowed to 23,247 results for: crispr

1. pCpf1-sp

   Plasmid

   #122186

   Purpose

   Bacterial genome editing plasmid with spectinomycin resistance marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLH-stsgRNA3.1

   Plasmid

   #64118

   Purpose

   Vector for expression of St1 sgRNA3.1 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   St1 sgRNA3.1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBbS8c-ddcpf1-rfp

   Plasmid

   #153038

   Purpose

   For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E993A

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p2CT-His-MBP-Lse_C2c2_WT

   Plasmid

   #83486

   Purpose

   Bacterial expression of WT L. seeligeri C2c2 CRISPR effector

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lse_C2c2_WT
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pC0045-EF1a-PguCas13b-NES-HIV

   Plasmid

   #103861

   Purpose

   Expresses PguCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PguCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBx-Spas-sgRNA-Kan

   Plasmid

   #105233

   Purpose

   Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NAX_CAGGS_CAS9_m4_KRAB_mcherry

   Plasmid

   #167878

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

8. p2CT-His-MBP-Lsh_C2c2_WT

   Plasmid

   #83487

   Purpose

   Bacterial expression of WT L. shahii C2c2 CRISPR effector

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lsh_C2c2_WT
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHAGE-EFS-dCas9-GFP

   Plasmid

   #64104

   Purpose

   Expression of Sp dCas9-GFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Sp dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-PEmax-SpG-P2A-hMLH1dn

    Plasmid

    #211550

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG-P2A-hMLH1dn
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pVDM1001

    Plasmid

    #134660

    Purpose

    CRISPR delivery and repair template delivery vector

    Depositor

    Willem van Schaik

    Article

    de Maat et al FEMS Microbiol Lett. 2019 Nov 1;366(22).

    Insert

    CRISPR guide RNA array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRU324

    Plasmid

    #167691

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pC0044-EF1a-RanCas13b-NES-mapk

    Plasmid

    #103855

    Purpose

    Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    RanCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and mapk NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRU319

    Plasmid

    #167686

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS143

    Plasmid

    #122377

    Purpose

    CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBbS8c-ddcpf1-Δ

    Plasmid

    #153039

    Purpose

    For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E993A

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCFD2-dU6:2gRNA

    Plasmid

    #49409

    Purpose

    expression of gRNA under control of the Drosophila U6:2 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-2:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-2

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HP138-puro

    Plasmid

    #134246

    Purpose

    Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Puro selection.

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    Contains Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330A_D10A-1x5

    Plasmid

    #58775

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCFD1-dU6:1gRNA

    Plasmid

    #49408

    Purpose

    expression of gRNA under control of the Drosophila U6:1 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-1:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-1

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge