We narrowed to 5,064 results for: cre encoding plasmid

1. pAAV-TRE-DIO-eYFP-f

   Plasmid

   #104057

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   EYFP-f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-pEF1a-DIO-tTAc

   Plasmid

   #172126

   Purpose

   Expression of InteinC-tTAC in Cre recombinase positive cells

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   InteinC-tTAc
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1_-DIO-Varnam1-WPRE

   Plasmid

   #240054

   Purpose

   Cre dependent Red fluorescent voltage indicator for mammalian expression

   Depositor

   Mark Schnitzer

   Article

   Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

   Insert

   VARNAM1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   July 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV[FLEX]-CAG-EGFP-pri-miR-137

   Plasmid

   #235159

   Purpose

   Expresses EGFP and miR-137 primary transcript in a Cre recombinase-dependent manner

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   EGFP/pri-miR-137 (Mir137 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO-GRAB_g5-HT2h

   Plasmid

   #208715

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons in the presence of Cre recombinase

   Depositor

   Yulong Li

   Article

   Deng et al bioRxiv 2023.05.27.542566

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT2h
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV1-DIO-eGFP-pvRPL10a

   Plasmid

   #202542

   Purpose

   Cr-dependent (DIO) expression of eGFP-tagged ribosomal subunit that uses the prairie vole RPL10a gene sequence under the human synapsin promoter.

   Depositor

   Zoe Donaldson

   Article

   Sadino et al Elife. 2023 Feb 28;12:e80517. doi: 10.75

   Insert

   RPL10a subunit of the ribosome
   

   Use

   AAV and Cre/Lox

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   optimized for prairie vole DNA sequence and optim…

   Promoter

   human synapsin

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAGGS-Flex/3'USS(103)-GFP

   Plasmid

   #197890

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAGGS-Flex/3'USS(553)-GFP

   Plasmid

   #197888

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAGGS-Flex/3'USS(285)-GFP

   Plasmid

   #197889

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCALNL-tdTomato-2A-mSyp::EGFP

    Plasmid

    #191213

    Purpose

    Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

    Depositor

    Theofanis Karayannis

    Article

    Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

    Insert

    tdTomato-T2A-mSyp::EGFP (Syp Mouse)
    

    Use

    Pcagen

    Tags

    mSyp::EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

    Plasmid

    #184636

    Purpose

    Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Gat1 gene with Arginine 69 changed to Lysine, to …

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP]

    Plasmid

    #153542

    Purpose

    AAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeQ-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α1.1

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1α1.1-FLEX-rc [ChromeT-GFP]

    Plasmid

    #153541

    Purpose

    AAV-mediated expression of ChromeT-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α1.1

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTCO2-mTDGi.2

    Plasmid

    #149431

    Purpose

    miniTdg gene, SUMOylation mutant

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    Tdg (Tdg Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    K341A SUMOylation mutant, SNPs of Tdg gene in OLA…

    Promoter

    Tdg promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTCO2-mTDGi.1

    Plasmid

    #149430

    Purpose

    miniTdg gene, catalytic mutant

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    Tdg (Tdg Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    N151A catalytic mutant, SNPs of Tdg gene in OLA/1…

    Promoter

    Tdg promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-SARS-CoV-2 S1 RBD

    Plasmid

    #167013

    Purpose

    Gateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2

    Depositor

    Spyros Petrakis

    Article

    Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    No stop codon

    Promoter

    Available since

    April 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid

    #107941

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R)
    

    Use

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R and K548R

    Promoter

    CAG

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-fDIO-Cre (AAV Retrograde)

    Viral Prep

    #121675-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Alexander Nectow, Esteban Engel

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Tags

    None

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

19. pAAV2-CAGIG-WGA-mCherry

    Plasmid

    #224119

    Purpose

    To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.

    Depositor

    Pantelis Tsoulfas

    Insert

    WGA-mCherry
    

    Use

    AAV

    Tags

    WGA and mCherry

    Expression

    Mammalian

    Mutation

    All mutations related to the WGA in this construc…

    Promoter

    CAGIG

    Available since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-link-GFPγ-SpHis5

    Plasmid

    #60781

    Purpose

    Yeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selection

    Depositor

    Linda Huang

    Article

    Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

    Insert

    GFPγ
    

    Use

    Yeast integrating

    Tags

    Expression

    Mutation

    T65C S72A V163A I167T L231H

    Promoter

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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