We narrowed to 8,464 results for: gnal

1. hNFAT2-409:TALE:VP64:KRɸ

   Plasmid

   #160661

   Purpose

   Constitutive expression of a truncated version of hNFAT (aa 2-409) fused to the DNA-binding domain from TALEN1257, the VP64 activation domain and the membrane anchor peptide KRɸ.

   Depositor

   Roman Jerala

   Article

   Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

   Insert

   hNFAT2-409:TALE:VP64:KRɸ
   

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNDL-60

   Plasmid

   #161720

   Purpose

   In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

   Depositor

   Adam Cohen

   Article

   McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

   Insert

   cytosolic acvr2b-CIB1N (acvr2b Zebrafish)
   

   Use

   In vitro transcription

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRXG

   Plasmid

   #113643

   Purpose

   This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   mRFP1-linker-sfGFP
   

   Use

   Synthetic Biology

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK7-FLAG-TSC2

   Plasmid

   #8996

   Depositor

   John Blenis

   Article

   Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

   Insert

   TSC2 (TSC2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-ER-GCaMP3

   Plasmid

   #64854

   Purpose

   Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CYP450-GCaMP3
   

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

   Plasmid

   #227774

   Purpose

   Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Insert

   loxP-dsR-loxP
   

   Use

   CRISPR

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. NbV5-HALOTAG

   Plasmid

   #201482

   Purpose

   Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-HALOTAG
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-1xHA CARM1 WT

   Plasmid

   #81118

   Purpose

   mammalian expression of CARM1 WT

   Depositor

   Sung Hee Baek

   Article

   Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

   Insert

   CARM1 (Carm1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCMV Fcgamma RIIa IRES neo

   Plasmid

   #14948

   Depositor

   Axel Nohturfft

   Article

   Trivedi et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

   Insert

   Fcgamma RIIa (FCGR2A Human)
   

   Expression

   Mammalian

   Available Since

   May 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C1-Rheb

    Plasmid

    #133767

    Purpose

    Expresses GFP-tagged Rheb in mammalian cells

    Depositor

    Shawn Ferguson

    Article

    Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

    Insert

    Rheb (RHEB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCherry-mNrp1

    Plasmid

    #21934

    Depositor

    Guido Serini

    Article

    Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

    Insert

    neuropilin 1 (Nrp1 Mouse)
    

    Tags

    Cherry and His

    Expression

    Mammalian

    Available Since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pNTI934

    Plasmid

    #216637

    Purpose

    Cal-ID-PM (V5) Plasma membrane-localized Cal-ID, mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    Cal-ID-PM
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCLX-CHOP-dGFP

    Plasmid

    #71299

    Purpose

    Lentiviral plasmid to express destabilized GFP under the control of the ATF-responsive CHOP promoter

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    dGFP
    

    Use

    Lentiviral

    Promoter

    CHOP (ATF-responsive)

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag pLJM1 RagB 54L

    Plasmid

    #19314

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding B (RRAGB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T54L

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. GRK2-CAAX

    Plasmid

    #166224

    Purpose

    GRK2-CAAX mammalian expression plasmid

    Depositor

    Robert Lefkowitz

    Article

    Inglese et al Nature. 1992 Sep 10;359(6391):147-50. do

    Insert

    bovine GRK2-CAAX (GRK2 Bovine)
    

    Tags

    no

    Expression

    Mammalian

    Mutation

    adding CAAX sequence at C terminal

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH_Str-KDEL_neomycin

    Plasmid

    #65307

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Use

    Lentiviral

    Tags

    KDEL motif and Signal Sequence

    Promoter

    CMV

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNTI821

    Plasmid

    #216630

    Purpose

    Tet-inducible Cal-ID (V5) for Sleeping Beauty transposon system

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    Cal-ID
    

    Use

    Sleeping beauty transposon system

    Tags

    V5

    Expression

    Mammalian

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLck-mVenus-C1

    Plasmid

    #84337

    Purpose

    Expresses a mVenus tagged with a membrane localization sequence

    Depositor

    Dorus Gadella

    Article

    van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

    Insert

    Lck (MGCVCSSNPELPVAT)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiPGK Puro DEST JNKKTRrsGreenF-E

    Plasmid

    #205764

    Purpose

    Expression of JNK KTR rsGreenF-E under PKG promoter (With Puromycin Resistance)

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    JNK KTR rsGreenF-E
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCMV Puro DEST ERKKTRrsFastLime

    Plasmid

    #205765

    Purpose

    Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    ERK KTR rsFastLime
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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