We narrowed to 12,568 results for: 109
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Plasmid#163412PurposeExpresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
UseTagsExpressionMammalianMutationThe C-terminal tail domain (530-1038 aa) is delet…PromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C427A-2xStrep
Plasmid#131109PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C427A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
UseTags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 427 muta…PromoterCMVAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_CH2charge
Plasmid#105858PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
UseTagsExpressionMammalianMutationmutated heavy chain of mouse IgG3, muatations: Hi…PromoterhEF1-HTLV promAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL4D
Plasmid#67468PurposeMammalian expression of hArl4D with stop codon (native expression)DepositorInsertARL4D (ARL4D Human)
UseTagsExpressionMammalianMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL4D-V5-His6
Plasmid#67447PurposeMammalian expression of hArl4D with C-term V5 and His6 tagDepositorInsertARL4D (ARL4D Human)
UseTagsHis and V5ExpressionMammalianMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL4D-V5-His6
Plasmid#67426PurposeExpression of hArl4D with C-term V5 and His6 tag in E. coliDepositorInsertARL4D (ARL4D Human)
UseTagsHis and V5ExpressionBacterialMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL4D-GFP
Plasmid#67405PurposeMammalian expression of hArl4D with C-term GFP tagDepositorInsertARL4D (ARL4D Human)
UseTagsGFPExpressionMammalianMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL4D-no stop
Plasmid#67337PurposehArl4D without stop codon in Gateway Entry vectorDepositorInsertARL4D (ARL4D Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARF4-no stop
Plasmid#67311PurposehArf4 without stop codon in Gateway Entry vectorDepositorInsertARF4 (ARF4 Human)
UseGateway entry vectorTagsExpressionBacterialMutationV53A, L123P and M134I mutations were unintended P…PromoterAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
I-GCAG
Plasmid#43109DepositorInsertI-GCAG
UseBacterial cloningTagsExpressionMutationPromoterAvailable sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
UseTagsHalo and His6ExpressionBacterial, Insect, and Mamm…MutationPromoterT7 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX458-Dual-Guide-Donor-ShuttleOnly
Plasmid#171098PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationXbaI site added upstream of U6 promotor. GtoC mu…PromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-EGFP-PARP1
Plasmid#176146PurposeEGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassetteDepositorInsertPoly(ADP-Ribose) Polymerase 1 (PARP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FGFR4-V5/HIS
Plasmid#201109Purposeexpression of human FGFR4 receptor tyrosine kinase in mammalian cellsDepositorInserthuman FGFR4 receptor tyrosine kinase, full length, wildtype (FGFR4 Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-BRCT2-Linker-eGFP
Plasmid#176085PurposeEGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassetteDepositorInsertBRCT2 (XRCC1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-BRCT1-Linker-eGFP
Plasmid#176074PurposeEGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassetteDepositorInsertBRCT1 (XRCC1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCasper4-g-osk-TRICK
Plasmid#71244Purposeoskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.DepositorInsertoskar (osk Fly)
UseP element-mediated germline transformationTags12xPP7 and 6xMS2ExpressionInsectMutationPromoteroskAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-OGDH_wobble
Plasmid#110938PurposeOGDH ORF containing silent wobble mutations insensitive to corresponding shRNAsDepositorInsertOGDH wobble cDNA (OGDH Human)
UseLentiviralTagsExpressionMutation15 silent wobble mutations corresponding to three…PromoterSV40Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-vsfGFP-0
Plasmid#191097PurposeTo express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-vsfGFP-0
UseAAVTagsGreen fluorescent protein bound to enhancer nanob…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(Y40C)
Plasmid#164903PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.DepositorInsertRaichuEV-Rac1 (RAC1 Human)
UseTagsExpressionMammalianMutationY40C mutation in the Rac1 domain whcih make the s…PromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only