Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 4,275 results for: Abo

Showing: 2301 - 2320 of 4275 results

  2. pCHC023 (∆MBH)

    Plasmid
    #226201
    Purpose
    Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for membrane bound hydrogenase operon
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    ∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226201-plasmid-map-sequence-id-453504

    Enlarge

  5. pCHC027 (∆pemK)

    Plasmid
    #226204
    Purpose
    Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Upstream/downstream homology arms for addiction system toxinPemK.
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226204-plasmid-map-sequence-id-452438

    Enlarge

  10. pACB143

    Plasmid
    #222214
    Purpose
    For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    vanB(A24P)
    Use
    Tags
    N-terminal, thrombin-cleavable 6x His tag
    Expression
    Bacterial
    Mutation
    Changed Alanine 24 to Proline
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222214-plasmid-map-sequence-id-438176

    Enlarge

  12. pACB109

    Plasmid
    #222208
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA (K10N)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Lys10 to Asn
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222208-plasmid-map-sequence-id-437369

    Enlarge

  13. pACB110

    Plasmid
    #222209
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA (G258D)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Gly258 to Aspartate
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222209-plasmid-map-sequence-id-437372

    Enlarge

  14. pACB111

    Plasmid
    #222210
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(G76V)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed glycine 76 to valine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222210-plasmid-map-sequence-id-437376

    Enlarge

  15. pACB112

    Plasmid
    #222211
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(S11R)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Serine 11 to Arginine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222211-plasmid-map-sequence-id-437378

    Enlarge

  16. pACB113

    Plasmid
    #222212
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Article
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(W15C)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Tryptophan 15 to Cysteine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222212-plasmid-map-sequence-id-437381

    Enlarge

Showing: 2301 - 2320 of 4275 results