We narrowed to 4,454 results for: TIL

1. Plasmid EUPp_09

   Plasmid

   #157745

   Purpose

   PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

   Depositor

   Kang Zhou

   Article

   Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

   Inserts

   acetaldehyde dehydrogenase
   alcohol dehydrogenase (ADH2 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PgyrA

   Available since

   June 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-TAG-ERN

   Plasmid

   #80476

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b_Q69L

   Plasmid

   #186272

   Purpose

   Expression vector of His-RanQ69L in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Use

   Tags

   His-tagged at N terminal

   Expression

   Bacterial

   Mutation

   Changed Q69 into L

   Promoter

   T7 promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIC291

   Plasmid

   #51403

   Purpose

   Retroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Article

   Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

   Insert

   Ska1 IMAGE 3909951 (SKA1 Human)
   

   Use

   Retroviral

   Tags

   EGFP, S tag, and TEV protease site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRetroX-PTuner DD-linker-M.EcoGII

   Plasmid

   #122082

   Purpose

   Express M.EcoGII with detabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of M.EcoGII

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVUHshp53

   Plasmid

   #11653

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   GFP, shRNA against TP53 (TP53 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b_RanWT

   Plasmid

   #186270

   Purpose

   Expression vector of His-RanQ69L in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Use

   Tags

   His tagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LC001: pMVP/PB/Hygro-DEST

   Plasmid

   #121876

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-S in pAR9 (rep/cap)

    Plasmid

    #174539

    Purpose

    encodes AAV-S capsid for efficient gene delivery to the mammalian cochlea

    Depositor

    Casey Maguire

    Article

    Ivanchenko et al Mol Ther Methods Clin Dev. 2021 Mar 29;2

    Insert

    VP1 of AAV9 containing the AAV-S 7-mer peptide insert
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

    Plasmid

    #125228

    Purpose

    Expresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. NAX_CAGGS_CAS9_m4_VPR_EBFP2

    Plasmid

    #167881

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-RT004-LaG30VB

    Plasmid

    #163384

    Purpose

    2nd generation lentiviral vector to express anti-GFP(LaG30) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG30-VEEGFRtm-BFP (LaG30VB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a-cys-His6-OtUBD

    Plasmid

    #190091

    Purpose

    Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    OtUBD
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP-Rab4a-7

    Plasmid

    #56440

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pMuLE ENTR MCS R4-R3

    Plasmid

    #62086

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMuLE ENTR MCS L1-L4

    Plasmid

    #62087

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFPC1-hMyoX

    Plasmid

    #47608

    Depositor

    Emanuel Strehler

    Article

    Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

    Insert

    MyoX (MYO10 Human)
    

    Use

    Tags

    EGFP-HA-His

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-EF1Alpha-Tet-on 3G

    Plasmid

    #118592

    Purpose

    Expresses Tet-on 3G from the EF1Alpha promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Tet-on 3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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