We narrowed to 2,383 results for: mia

1. pLV-tetO-Ets2

   Plasmid

   #70272

   Purpose

   Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ets2

   Depositor

   Hubert Schorle

   Article

   Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

   Insert

   Ets2 (Ets2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX020

   Plasmid

   #167146

   Purpose

   MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   fungal caffeoylpiruvate hydrolase, nnCPH
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-flex-TVA

   Plasmid

   #69618

   Purpose

   Express recepter TVA for envA with AAV

   Depositor

   Naoshige Uchida

   Article

   Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

   Insert

   Avian sarcoma and leukemia virus receptor 950 (CD320 quail, Synthetic)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX131

   Plasmid

   #167149

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   4'-phosphopantetheinyl transferase, npgA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CD8a-hnRNP-M-EGFP

   Plasmid

   #86054

   Purpose

   A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   CD8a (CD8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CD8a luminal and transmembrane domians along with…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX018

   Plasmid

   #167144

   Purpose

   MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   fungal luciferase, nnLuz
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX019

   Plasmid

   #167145

   Purpose

   MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   fungal hispidin-3-hydroxylase, nnH3H
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB9P3

   Plasmid

   #210034

   Purpose

   Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   prime editor 2
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+-FLAG3-drN-hsAgo2

   Plasmid

   #221724

   Purpose

   Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.

   Depositor

   David Bartel

   Article

   Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

   Insert

   drN-hsAgo2 (AGO2 Human)
   

   Use

   Fish expression

   Tags

   3xFLAG

   Expression

   Mutation

   Swap of N-domain for zebrafish Ago2 N-domain

   Promoter

   simian CMV IE94

   Available since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+-FLAG3-hsN-drAgo2

    Plasmid

    #221717

    Purpose

    Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsN-drAgo2 (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    Swap of N-domain for human Ago2 N-domain

    Promoter

    simian CMV IE94

    Available since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+-FLAG3-hsAgo2EF-DY

    Plasmid

    #221723

    Purpose

    Expression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsAgo2EF-DY (AGO2 Human)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Expression

    Mutation

    E637D and F666Y in active site

    Promoter

    simian CMV IE94

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNK7191

    Plasmid

    #219764

    Purpose

    MoClo-compatible Level M for expression PpASCL in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PpASCL
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX022

    Plasmid

    #167148

    Purpose

    MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    C-part of fungal hispidin synthase, nnHispS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX021

    Plasmid

    #167147

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    N-part of fungal hispidin synthase, nnHispS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PEmax

    Plasmid

    #174820

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PEA1-Puro

    Plasmid

    #171991

    Purpose

    Delivers all prime editing nickase components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. PEA1-GFP

    Plasmid

    #171993

    Purpose

    Delivers all prime editing nickase components in a single, GFP selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-PEmax for IVT

    Plasmid

    #178113

    Purpose

    Template for in vitro transcription of PEmax

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mutation

    Detailed in manuscript

    Promoter

    T7 (inactivated)

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastbac1-Flag-FANCD2

    Plasmid

    #134904

    Purpose

    codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    FANCD2 (FANCD2 Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    polyhedron

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+HyPer7-NLS

    Plasmid

    #136468

    Purpose

    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Triple nuclear localization signal of SV40 (simia…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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