We narrowed to 4,418 results for: 1-Oct;
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Plasmid#117667PurposeStudy secretion efficiency of Btl2DepositorInsertpre-Ost1-alphaf(I)-Btl2
ExpressionYeastMutationThis plasmid encodes the lipase Btl2 together wit…PromoterpAOX1Available SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ORP8-H514A-H515A
Plasmid#208360PurposeMammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8DepositorAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
DiLiCre 2.0
Plasmid#198752PurposeA Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertCre-PhoCI-ERT2-ERT2
UseLentiviralTagsCre, kept in a photocleavable site, followed by 2…ExpressionBacterial and MammalianPromoterTRE3GVAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-3FLAG-RAB11A
Plasmid#176489PurposeFor lentiviral transduction of 3FLAG-RAB11ADepositorAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
UbC-blasticidine-P2A-TETon-3G transactivator
Plasmid#200191PurposeA TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertblasticidine - P2A - TETon-3G transactivator
UseLentiviralExpressionBacterial and MammalianPromoterUbCAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-GRAM-H
Plasmid#211706PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cellsDepositorInsertB-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
TagsddFP-B3ExpressionMammalianMutationChanged Glycine 187 to LeucinePromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
B-GRAM-W
Plasmid#211707PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cellsDepositorInsertB-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
TagsddFP-B3ExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1
Plasmid#187454PurposeCoselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmidDepositorInsertATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII
Plasmid#225075PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIII)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII-43
Plasmid#225077PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIII-43)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII
Plasmid#225066PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
PM-RA
Plasmid#211705PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cellsDepositorAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-3FLAG-TFR1
Plasmid#176490PurposeFor lentiviral transduction of 3FLAG-TFR1DepositorAvailable SinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-hMLH1NTD-NLS
Plasmid#174826PurposeMammalian expression of human MLH1NTD-NLS (codon optimized)DepositorInserthuman MLH1_NTD-NLS (codon optimized)
TagsSV40 bpNLSExpressionMammalianMutationtruncation of residues 1-335PromoterEF1aAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW2661_pCAG-PV1-iCre-N270-L1-ABI-NLS-BGHpA
Plasmid#108745PurposeExpresses N-terminus to aa270 of Cre recombinase fused to ABI CIDDepositorInsertCre recombinase fragment N-terminus to aa270
UseCre/Lox and Synthetic BiologyTagsABI,NLSExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpA
Plasmid#108746PurposeExpresses aa271 to C-terminus of Cre recombinase fused to PYL CIDDepositorInsertCre recombinase fragment aa271 to C-terminus
UseCre/Lox and Synthetic BiologyTagsNLS and PYLExpressionMammalianPromoterpCAGAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7-p53DD-pcDNA3
Plasmid#25989DepositorInserttumor protein p53 (TP53 Human)
TagsT7ExpressionMammalianMutationtruncated cDNA encodes truncated protein (aa 300-…Available SinceOct. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-Wnt3a
Plasmid#114287PurposeTET-inducible expression of Wnt3aDepositorInsertWnt3a (Wnt3a Mouse)
UseLentiviralAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BTB+BACK)
Plasmid#169723PurposeMammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)DepositorInsertSPOP (Spop Mouse)
TagsV5ExpressionMammalianMutationL186D, L190D, L193D, I217K, Y353EPromoterCMVAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPOP-V5 (BTB)
Plasmid#169721PurposeMammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)DepositorInsertSPOP (Spop Mouse)
TagsV5ExpressionMammalianMutationL186D, L190D, L193D, I217KPromoterCMVAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only