We narrowed to 5,084 results for: CAPS

1. PCR4-beta-globin::lacZ-H11

   Plasmid

   #227000

   Purpose

   enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains human beta-globin minimal promoter)

   Depositor

   Guillaume Andrey

   Article

   Darbellay et al Nat Commun. 2024 Jun 6;15(1):4820. doi:

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   beta-globin

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpdCas9 KRAB

   Plasmid

   #188499

   Purpose

   Expresses FLAG tagged SpdCas9 KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1-Luciferase

   Plasmid

   #99283

   Purpose

   All-in-one lentivector with tet-inducible luciferase

   Depositor

   Stephen Tapscott

   Article

   Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_GEM4-Halo-FRB

   Plasmid

   #197057

   Purpose

   Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_GEM7-Halo-FRB

   Plasmid

   #197058

   Purpose

   Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS2118

   Plasmid

   #49143

   Purpose

   AAV plasmid with smCBA promoter driving expression of iCre. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Mirjalili Mohanna et al Gene Ther. 2023 Apr 19. doi: 10.1038/s41

   Insert

   ssAAV-smCBA-iCre-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   smCBA

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKL001

   Plasmid

   #98917

   Purpose

   Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

   Insert

   CaPR
   

   Expression

   Bacterial

   Promoter

   BBa_J23118 - Strong Constitutive

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. BFP-Dcp1a

   Plasmid

   #153973

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1a

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA decapping enzyme 1a (DCP1A Human)
   

   Tags

   mTagBFP-c1

   Expression

   Mammalian

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-Flavidin

   Plasmid

   #89881

   Purpose

   Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

   Depositor

   Mark Howarth

   Article

   Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

   Insert

   Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
   

   Tags

   6 glutamate tag (C terminal on insert)

   Expression

   Bacterial

   Mutation

   K80R, K121R, K132R, K134R

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Burden Monitor lambda version

    Plasmid

    #66073

    Purpose

    The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

    Insert

    GFP
    

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pUCXMG

    Plasmid

    #204409

    Purpose

    Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

    Depositor

    David Ackerley

    Article

    Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-hXRCC1

    Plasmid

    #70759

    Purpose

    bacterial expression of human XRCC1

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    XRCC1 (XRCC1 Human)
    

    Tags

    6-HIS

    Expression

    Bacterial

    Promoter

    T7/lac operon

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-TO-Vpr-mRuby3-IN

    Plasmid

    #167584

    Purpose

    For producing fluorescently labeled HIV-1; expresses HIV-1 integrase fused to Vpr for packaging into HIV-1 and the fluorophore mRuby3

    Depositor

    Zandrea Ambrose

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    Vpr-mRuby3-IN
    

    Tags

    Vpr-mRuby3

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-IP-PEX3-EGFP

    Plasmid

    #228142

    Purpose

    Expresses PEX3-GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    PEX3 (PEX3 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-Rac1-DN

    Plasmid

    #109582

    Purpose

    Multisite gateway vector for 3' tagging with dominant negative Rac1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rac1 (rac1a Zebrafish)
    

    Use

    Zebrafish plasmids

    Mutation

    dominant negative

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKC518 pBABE EGFP-LIN52

    Plasmid

    #192697

    Purpose

    Retroviral transgene expression of human LIN52

    Depositor

    Iain Cheeseman

    Article

    Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

    Insert

    LIN52 (LIN52 Human)
    

    Use

    Retroviral

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS613_pTetR-P2A-BFPnls/sgTelo

    Plasmid

    #108649

    Purpose

    Telomere-targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Telomere-targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5 bovine GRK2

    Plasmid

    #14691

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

    Insert

    GRK2 (GRK2 Bovine)
    

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

19. p3E-Rac1

    Plasmid

    #109581

    Purpose

    Multisite gateway vector for 3' tagging with Rac1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rac1 (rac1a Zebrafish)
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. actc1b-mKate2-Rac1

    Plasmid

    #109529

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to Rac1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rac1 (rac1a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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