We narrowed to 152,634 results for: des
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Plasmid#168251Purpose"label active Rac under Rac2 neutrophil-specific knockout background"DepositorInsertPBD GFP
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides
Plasmid#168254Purpose"label stable F-actin under Rac2 neutrophil-specific knockout background"DepositorInsertUtrophin-GFP
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-redesigned-human-Caspase-4_ANR261ENP_D315H_M318K_T352K
Plasmid#183357PurposeExpression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4 with ICE activity (CASP4 Human)
UseRetroviralTagsMycExpressionMutationANR261ENP, D315H, M318K, T352KPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-PBD-GFP-U6ac-ctrl guides
Plasmid#168252Purpose"label active Rac; control for neutrophil-specific knockout"DepositorInsertPBD GFP
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides
Plasmid#168253Purpose"label stable F-actin; control for neutrophil-specific knockout"DepositorInsertUtrophin-GFP
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
Plasmid#159838PurposeThree-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arraysDepositorInsertpDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (FL-based no force control)
Plasmid#118722PurposeThe donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y72L mutation in mCherry…PromoterCMVAvailable sinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS2
Plasmid#69228PurposeExpresses R1335K mutant SpCas9 fused to ZFP-TS2 in mammalian cellDepositorInsertTS2 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS3
Plasmid#69229PurposeExpresses R1335K mutant SpCas9 fused to ZFP-TS3 in mammalian cellDepositorInsertTS3 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-ZFP_TS4
Plasmid#69230PurposeExpresses R1335K mutant SpCas9 fused to ZFP-TS4 in mammalian cellDepositorInsertTS4 ZFP
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP
Plasmid#65846PurposeMammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorInsertTransmembrane (23 AA) of UQCC3 (UQCC3 Human)
UseTagsGFPExpressionMammalianMutationR5A-K6APromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP
Plasmid#65847PurposeMammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorInsertTransmembrane (23 AA) of UQCC3 (UQCC3 Human)
UseTagsGFPExpressionMammalianMutationR5D-K6DPromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2ala7-TEV-YFP-Flag
Plasmid#41013DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
UseTagsTEV-YFP-FlagExpressionMammalianMutationchanged amino acids 1189-1192 to AlaninePromoterAvailable sinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2ala5-TEV-YFP-Flag
Plasmid#41012DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
UseTagsTEV-YFP-FlagExpressionMammalianMutationchanged amino acids 1185-1188 to AlaninePromoterAvailable sinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2ala4-TEV-YFP-Flag
Plasmid#41011DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
UseTagsTEV-YFP-FlagExpressionMammalianMutationchanged amino acids 1183-1186 to AlaninePromoterAvailable sinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry
Plasmid#71282PurposeZebrafish expression vector for heat-shock inducible expression of APEX2-GBPDepositorInsertAPEX2-GBP
UseZebrafish expression with tol2TagsExpressionMutationPromoterHSP70lAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2
Plasmid#220508PurposeTGFb-inducible fluorescent reporter (TIE:EGFP)DepositorInsertsTGFb inducible enhancer
beta-globin minimal promoter
SV40 PolyA signal
UseZebrafish expression (tol2)TagsEGFPExpressionMutationPromoterAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I acceptor only control (F40-based tension sensor)
Plasmid#119189PurposeThe acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.DepositorInserthuman Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin I donor only control (F40-based tension sensor)
Plasmid#119188PurposeThe donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.DepositorInserthuman Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
UseTransposonTagsmTFP1-F40-mEYFPExpressionMammalianMutationinserted F40-based tension sensor module after aa…PromoterTCEAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)
Plasmid#192123PurposeLentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only