We narrowed to 4,489 results for: TIL;

1. pET28b_Q69L

   Plasmid

   #186272

   Purpose

   Expression vector of His-RanQ69L in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Tags

   His-tagged at N terminal

   Expression

   Bacterial

   Mutation

   Changed Q69 into L

   Promoter

   T7 promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-mouse vinculin full length (889)

   Plasmid

   #67935

   Purpose

   Mammalian expression of mouse vinculin with GFP fusion

   Depositor

   Alpha Yap

   Article

   Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

   Insert

   Vinculin (Vcl Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L159P in vinculin

   Promoter

   CMV

   Available Since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-GFP(S65T)-His3MX6

   Plasmid

   #41598

   Depositor

   John Pringle

   Article

   Longtine et al Yeast. 1998 Jul;14(10):953-61.

   Insert

   His3
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   May 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

   Plasmid

   #125235

   Purpose

   Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-N2c_envA-IRES-Neo

   Plasmid

   #172368

   Purpose

   Generation of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418)

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   envA + Neo
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRFP-NLS

   Plasmid

   #105296

   Purpose

   To visualize nuclear dynamics in living cells

   Depositor

   Kenneth Yamada

   Article

   Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

   Insert

   Rac1 (RAC1 Human)
   

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   aa encoding 172-192

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b_RanWT

   Plasmid

   #186270

   Purpose

   Expression vector of His-RanQ69L in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Tags

   His tagged

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. LC001: pMVP/PB/Hygro-DEST

   Plasmid

   #121876

   Purpose

   pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Piggybac transposon, pmvp gateway destination vec…

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CVS-N2c-mTurquoise

   Plasmid

   #172376

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   mTurquoise
   

   Use

   Neurotropic virus

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMuLE ENTR MCS R4-R3

    Plasmid

    #62086

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Feb. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. NAX_CAGGS_CAS9_m4_VPR_EBFP2

    Plasmid

    #167881

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MMLV-CAG-TVA-IRES-Puro

    Plasmid

    #172366

    Purpose

    Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + Puro
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PA-Cdc42

    Plasmid

    #75263

    Purpose

    Light-activated Cdc42

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    Cell Division Cycle 42 (CDC42 Human)
    

    Tags

    6xHIs, LOV2, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    F56W

    Promoter

    CMV

    Available Since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. LB601: pMVP/PB/eGFP-DEST

    Plasmid

    #121870

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-IRES-Gαi*-BERKY3

    Plasmid

    #158424

    Purpose

    BRET biosensor for detection of Gαi-GTP in lentiviral vector pLVX-IRES-Hyg

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Lentiviral

    Tags

    myc

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pMuLE ENTR MCS L1-L4

    Plasmid

    #62087

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a multiple cloning site. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Feb. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5 FRT/TO Plk4 ∆24-mCherry

    Plasmid

    #80271

    Purpose

    mammalian expression plasmid for Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2Apuro-Stargazin-mEGFP-iLID

    Plasmid

    #178529

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ePPEmax*

    Plasmid

    #205244

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOCC305

    Plasmid

    #118898

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and TwinStrep-MB…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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