We narrowed to 4,855 results for: U6...

1. pLV/Guide-mCherry

   Plasmid

   #170543

   Purpose

   Expresses mCherry and contains a cassette for U6 driven sgRNA transcription

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CBX3 A6.5 gRNA

   Plasmid

   #90569

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CBX3

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CBX3 (Guide Designation A6.5)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CBX3 A5.5 gRNA

   Plasmid

   #90568

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CBX3

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CBX3 (Guide Designation A5.5)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. piggyBAC-5xTAPE-1-BlastR

   Plasmid

   #175808

   Purpose

   Encodes 5xTAPE-1 construct that can be integrated using piggyBAC system

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Insert

   5xTAPE-1 construct
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330-PITCh-H2BC11

   Plasmid

   #207755

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. eSpCas9(1.1)-Puro

   Plasmid

   #117686

   Purpose

   The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

   Depositor

   Luc Leyns

   Article

   Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR

   Tags

   PuroR (R166H)

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   U6

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAtC

   Plasmid

   #78098

   Purpose

   Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance

   Depositor

   Jin-Soo Kim, Sang-Gyu Kim

   Article

   Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

   Plasmid

   #121944

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BRCA2 A11.2 gRNA

   Plasmid

   #90550

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BRCA2

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BRCA2 (Guide Designation A11.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RRM1 E1.3 gRNA

    Plasmid

    #90880

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM1 (Guide Designation E1.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAGK61

    Plasmid

    #228121

    Purpose

    Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #176272

    Purpose

    Expression of non-targeting sgRNA of CasMINI

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI non-targeting sgRNA (sgLacZ) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BPK1520_puroR

    Plasmid

    #173901

    Purpose

    SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

    Depositor

    Ronald Cohn

    Article

    Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/U6

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Adeno Cas9

    Plasmid

    #64072

    Purpose

    Adenoviral vector for the expression of the Cas9

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    U6 and CBh

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RRM1 E2.3 gRNA

    Plasmid

    #90881

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM1 (Guide Designation E2.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLL5.0 shFmnl2-EGFP

    Plasmid

    #187255

    Purpose

    Lentiviral expression of shRNA targeting Fmnl2

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

    Insert

    ShRNA for Fmnl2 (Fmnl2 Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RRM2 E4.3 gRNA

    Plasmid

    #90883

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM2 (Guide Designation E4.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RRM2 E3.3 gRNA

    Plasmid

    #90882

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM2 (Guide Designation E3.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV sgRNA Expression Plasmid

    Plasmid

    #174540

    Purpose

    Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

    Depositor

    Ormond MacDougald

    Article

    Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJY-RpABE

    Plasmid

    #112872

    Purpose

    binary vector for ABE expression in Arabidopsis

    Depositor

    Jin-Soo Kim, Jae-Young Yun

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA cassette
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge