We narrowed to 12,486 results for: mpl

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pCMV3TAG8li-hSTT3A-3FLAG

Plasmid

#159140

Purpose

Hu STT3A -flag mammalian expression vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

STT3 oligosaccharyltransferase complex catalytic subunit A (STT3A Human)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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shPBRM1 #122-Tet-pLKO-puro

Plasmid

#107401

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93-mut

Plasmid

#87335

Purpose

To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag Rab1a N124I

Plasmid

#46778

Purpose

expression of N124I Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (Rab1a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N124I cannot stably bind guanine nucleotide

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pD649-HAsp-PTPRF-Fc(DAPA)-AviTag-6xHis

Plasmid

#157036

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PTPRF (PTPRF Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ROBO2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156991

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ROBO2 (ROBO2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD19-Fc(DAPA)-AviTag-6xHis

Plasmid

#156714

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD19 (CD19 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD22-Fc(DAPA)-AviTag-6xHis

Plasmid

#156940

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD22 (CD22 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Plasmid

#159815

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes 1-480 WT

Plasmid

#158541

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Tags

V5

Expression

Mammalian

Mutation

Amino acids 1-480, C-terminal deletion

Promoter

CMV

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp13-HF_GC3opt

Plasmid

#157716

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Brg1 Del2 pET28a

Plasmid

#122116

Purpose

Expression Brg1 amino acids 1-837 in bacteria

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Brg1 (SMARCA4 Human)

Tags

6XHis and His-T7

Expression

Bacterial

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p2Flag CMV2-ZO-2-N

Plasmid

#27418

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus of ZO-2, so only…

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
pD649-HAsp-CD276-Fc(DAPA)-AviTag-6xHis

Plasmid

#156861

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD276 (CD276 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGLEC8-Fc(DAPA)-AviTag-6xHis

Plasmid

#156792

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC8 (SIGLEC8 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NCAM1-Fc(DAPA)-AviTag-6xHis

Plasmid

#156948

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NCAM1 (NCAM1 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGLEC10-Fc(DAPA)-AviTag-6xHis

Plasmid

#156906

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC10 (SIGLEC10 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nprot-HF_GC3opt

Plasmid

#157732

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nprot-HF_GC3opt (N SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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RMCE K>R DNA-PKcs

Plasmid

#233917

Purpose

Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

Depositor

Katheryn Meek

Article

Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

Insert

human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)

Expression

Mammalian

Mutation

K3753R

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGGD_MiniTurboID-YFP

Plasmid

#222434

Purpose

Golden Gate / Green Gate module for adding MiniTurboID and YFP as C-terminus of target protein.

Depositor

Dominique Bergmann

Article

Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

Insert

MiniTurboID (BirA mutant)

Use

Golden gate / green gate compatible cloning vector

Tags

Linker and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGGB_YFP-MiniTurboID

Plasmid

#222433

Purpose

Golden Gate / Green Gate module for adding YFP and MiniTurboID as N-terminus of target protein.

Depositor

Dominique Bergmann

Article

Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

Insert

MiniTurboID (BirA mutant)

Use

Golden gate / green gate compatible cloning vector

Tags

Linker and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA (MSW#111)

Plasmid

#208066

Purpose

expression of human Replication Protein A in E. coli

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)

Expression

Bacterial

Mutation

RPA32 has a point mutation Thr88(ACC) -> Ile(A…

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

Plasmid

#198750

Purpose

Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - SmallBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pODN-AAVS1-mNG-CAAX

Plasmid

#183870

Purpose

Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)

Use

CRISPR; Donor template

Tags

mNeonGreen-CAAX

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159809

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

Plasmid

#102421

Purpose

Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector

Depositor

Kian Peng Koh

Article

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Insert

Tet1 (Tet1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Modified at the Dharmacon SMARTpool siRNA #2 targ…

Promoter

TetO-CMV

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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VZV ORF 37(gH)

Plasmid

#64081

Purpose

eukaryotic expression plasmid for VZV glycoprotein H

Depositor

Randall Cohrs

Article

Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

Insert

VZV glycoprotein H

Expression

Mammalian

Promoter

CMV IE with intron

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
VZV ORF 60 (gL)

Plasmid

#64082

Purpose

eukaryotic expression plasmid for VZV glycoprotein L

Depositor

Randall Cohrs

Article

Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

Insert

VZV glycoprotein L

Expression

Mammalian

Promoter

CMV IE with intron

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pEGFPC1/GgVcl 1-851 A50I mutant

Plasmid

#46269

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-851 A50I mutation (VCL Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

1-851 (Vh) head domain, A50I mutation

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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