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Showing: 5901 - 5950 of 6656 results

  1. AICSDP-55:DMD-mEGFP

    Plasmid
    #159744
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Insert
    DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159744-plasmid-map-sequence-id-314374

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  11. sgRNA3_GAL4UAS-Luciferase reporter

    Plasmid
    #64159
    Purpose
    Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
    Depositor
    Moritoshi Sato
    Article
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Insert
    sgRNA3 for GAL4UAS-Luciferase reporter
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64159-plasmid-map-sequence-id-107444

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  13. pGen2.1-redCALWY-4

    Plasmid
    #50738
    Purpose
    Expresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.
    Insert
    gene for redCALWY-4 Zn2+ sensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50738-plasmid-map-sequence-id-76087

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  16. pGen2.1-redCALWY-1

    Plasmid
    #50739
    Purpose
    Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.
    Insert
    gene for redCALWY-1 Zn2+ sensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    50739-plasmid-map-sequence-id-76088

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  17. p-715MRPprom-MRPcucuc

    Plasmid
    #87199
    Purpose
    Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency
    Depositor
    Thomas Cech
    Article
    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:
    Insert
    RMRP (RMRP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GAGAG91-95 to CUCUC
    Promoter
    human RMRP
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    87199-plasmid-map-sequence-id-173709

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  20. jk107

    Plasmid
    #87007
    Purpose
    plasmid for cell culture expression of SYNseq components
    Depositor
    Anthony Zador
    Article
    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11
    Inserts
    SNAP-Nlg1AB-1xλN(i)
    mCherry
    Use
    Synthetic Biology; Sindbis virus genomic plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87007-plasmid-map-sequence-id-173032

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  23. pFGL1169R

    Plasmid
    #116899
    Purpose
    CenpC:mCherry (centromere marker)
    Depositor
    Naweed Naqvi
    Article
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Inserts
    Cenp C (partial)
    mCherry
    CenpC downstream region
    Use
    Fungal expression (in magnaporthe oryzae)
    Tags
    Expression
    Mutation
    truncated, no stop codon and without start codon
    Promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    116899-plasmid-map-sequence-id-232229

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  25. sgRNA1_GAL4UAS-Luciferase reporter

    Plasmid
    #64157
    Purpose
    Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
    Depositor
    Moritoshi Sato
    Article
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Insert
    sgRNA1 for GAL4UAS-Luciferase reporter
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64157-plasmid-map-sequence-id-107441

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  27. sgRNA2_GAL4UAS-Luciferase reporter

    Plasmid
    #64158
    Purpose
    Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
    Depositor
    Moritoshi Sato
    Article
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Insert
    sgRNA2 for GAL4UAS-Luciferase reporter
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64158-plasmid-map-sequence-id-107442

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  32. jk64

    Plasmid
    #87012
    Purpose
    genomic Sindbis virus plasmid for use in SYNseq PLA screen
    Depositor
    Anthony Zador
    Article
    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11
    Inserts
    Nrx1B-4xλN(i)
    mCherry
    Use
    Synthetic Biology; Sindbis virus genomic plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87012-plasmid-map-sequence-id-173035

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  36. pPWCh

    Plasmid
    #124238
    Purpose
    Gateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.
    Depositor
    Peter Vilmos
    Article
    Vilmos et al Cell Biol Int. 2016 Jun;40(6):696-707. d
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    UASp
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    124238-plasmid-map-sequence-id-240330

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  40. IDP429

    Plasmid
    #87016
    Purpose
    genomic Sindbis virus plasmid for use in SYNseq PLA screen
    Depositor
    Anthony Zador
    Article
    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11
    Inserts
    CLIP-Nrx1B-GFP-1xλN(c)
    mCherry
    Use
    Synthetic Biology; Sindbis virus genomic plasmid
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87016-plasmid-map-sequence-id-169748

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  42. pJC.15A.sgRNA.TA

    Plasmid
    #232483
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.
    Depositor
    James Collins
    Article
    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0
    Inserts
    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P3 constitutive promoter and native promoter
    Available since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232483-plasmid-map-sequence-id-466415

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  46. pACBE-FSR (pXK-1648)

    Plasmid
    #208821
    Purpose
    Universal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.
    Depositor
    Kun Xu
    Article
    Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi
    Type
    Empty backbone
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, U6
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208821-plasmid-map-sequence-id-417246

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Showing: 5901 - 5950 of 6656 results