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eIF3e- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192239

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3e

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)

Expression

Mammalian

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192242

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3f

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k sgRNA-2 PX459 plasmid

Plasmid

#192256

Purpose

expressing sgRNA-2 targeting eIF3k locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k sgRNA-1 PX459 plasmid

Plasmid

#192255

Purpose

expressing sgRNA-1 targeting eIF3k locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f sgRNA-1 PX459 plasmid

Plasmid

#192253

Purpose

expressing sgRNA-1 targeting eIF3f locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3f locus closed to stop coden (EIF3F Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f sgRNA-2 PX459 plasmid

Plasmid

#192254

Purpose

expressing sgRNA-2 targeting eIF3f locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b sgRNA-2 PX459 plasmid

Plasmid

#192250

Purpose

expressing sgRNA-2 targeting eIF3b locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b sgRNA-1 PX459 plasmid

Plasmid

#192249

Purpose

expressing sgRNA-1 targeting eIF3b locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC16A8_STOP

Plasmid

#161125

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A8 (SLC16A8 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pETDuet_huCAD_ICADL (TevS)

Plasmid

#100098

Purpose

dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

Depositor

Jim Wells

Article

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

DNA fragmentation factor subunit alpha (CAD, DFFA Human)

Expression

Bacterial

Mutation

two caspase cleavage sites (DETD-117 and DAVD-224…

Promoter

T7

Available Since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRA1-MYB

Plasmid

#141120

Purpose

Lentiviral overexpression of MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pmiRA1-MYC

Plasmid

#141121

Purpose

Lentiviral overexpression of MYC in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-MTCH2_STOP

Plasmid

#161120

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MTCH2 (MTCH2 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA5_C12orf49-miniTurbo

Plasmid

#155112

Purpose

transfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

C12orf49 (SPRING1 Human)

Tags

3x Flag and miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_EIF4E_WT

Plasmid

#82112

Purpose

Gateway Donor vector containing EIF4E , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EIF4E (EIF4E Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC32A1

Plasmid

#132112

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC32A1 (SLC32A1 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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eIF3f- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192241

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3f

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3f-knockin-homology arm-mAID-mClover-NeoR (EIF3F Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD7-COMP5AP-AviTag-9xHis

Plasmid

#157112

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD7 (CD7 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3a- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192236

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3a

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3a-knockin-homology arm-mAID-mCherry2-Hygro (EIF3A Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eIF3a- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192235

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3a

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3a-knockin-homology arm-mAID-mClover-NeoR (EIF3A Human)

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge