We narrowed to 5,086 results for: CAPS;

1. actb2-SecAnxaV-mKate2-acry-EGFP

   Plasmid

   #123628

   Purpose

   Ubiquitous zebrafish expression vector containing secreted human AnnexinV tagged to mKate2. Parton lab clone BDN.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   AnnexinA5
   

   Tags

   mKate2

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SG3ΔENV RT D185A

   Plasmid

   #145782

   Purpose

   Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).

   Depositor

   Wesley Sundquist

   Article

   Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

   Insert

   Reverse Transcriptase
   

   Use

   Lentiviral and Retroviral

   Expression

   Mammalian

   Mutation

   Changed Reverse Transcriptase Aspartic Acid 185 t…

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL057

   Plasmid

   #177169

   Purpose

   Expresses a nuclease dead MRE11 in human cells

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   MRE11 H129N (MRE11 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   nuclease-inactivating mutation (H129N)

   Promoter

   EF1a

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GRK2 S670A

   Plasmid

   #45755

   Depositor

   Robert Lefkowitz

   Article

   Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

   Insert

   GRK2 S670A (GRK2 Bovine)
   

   Expression

   Mammalian

   Mutation

   S670A

   Promoter

   CMV

   Available Since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

5. Burden Monitor phi80 version

   Plasmid

   #66074

   Purpose

   The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

   Insert

   GFP
   

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-mKate2-KDEL-pA

   Plasmid

   #109546

   Purpose

   Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CANE-LV envelope

   Plasmid

   #86666

   Purpose

   pCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirus

   Depositor

   Fan Wang

   Article

   Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

   Insert

   CANE-LV envelope
   

   Use

   Lentiviral, Mouse Targeting, and Synthetic Biolog…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

8. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

   Plasmid

   #129708

   Purpose

   Express cre-dependent KASH-EGFP and scramble shRNA

   Depositor

   Yi Zhang

   Article

   Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

   Insert

   KASH-EGFP
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pet28a-His6-Halo-Tev-Keap1

   Plasmid

   #62456

   Purpose

   His6-halo tagged human Keap1 protein for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Tags

   His-Halo-Tev

   Expression

   Bacterial

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRX-IPU-mTagBFP2-Sec61B

    Plasmid

    #228136

    Purpose

    Expresses mTagBFP2-Sec61B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Sec61B (SEC61B Human)
    

    Use

    Retroviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBbB8k-csg-amylase

    Plasmid

    #166859

    Purpose

    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.

    Depositor

    Neel Joshi

    Article

    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469

    Insert

    csgBACEFG, with α-amylase fused to CsgA via flexible linker
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

    Plasmid

    #129706

    Purpose

    Express cre-dependent KASH-EGFP and Gmeb1 shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IBU-SNAP-Rab7

    Plasmid

    #228141

    Purpose

    Expresses SNAP-Rab7 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Rab7 (Rab7 Mouse)
    

    Use

    Retroviral

    Tags

    SNAP

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE-neo largeTcDNA K1

    Plasmid

    #8581

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    E107K. Alteration in pRB consensus binding motif…

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

15. His-TEV-Aurein1.2_(+36GFP)-LPETG-His

    Plasmid

    #71755

    Purpose

    His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    Aurein1.2_(pos36_GFP)-LPETG-His
    

    Tags

    6XHis, Sortase (LPETG), and TEV Protease

    Expression

    Bacterial

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 GRK2 S670D

    Plasmid

    #45756

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

    Insert

    GRK2 S670D (GRK2 Bovine)
    

    Expression

    Mammalian

    Mutation

    S670D

    Promoter

    CMV

    Available Since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pFastBac1-OSF-TRIM5alpha(human)

    Plasmid

    #79034

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(human) (TRIM5 Human)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-Tight-Puro_ST18

    Plasmid

    #64844

    Purpose

    A lentiviral vector for the expression of mouse ST18 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    ST18 (St18 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_GEM22-Halo-FRB

    Plasmid

    #197059

    Purpose

    Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHYP-NPC-10H

    Plasmid

    #162789

    Purpose

    SARS-CoV-2 Nucleocapsid Protein expression in E.coli

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771

    Insert

    Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    10xHis

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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