We narrowed to 42,693 results for: LAS

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qTAG-C-dTAG-Blast

Plasmid

#207716

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pPE4max-Blasticidin

Plasmid

#194905

Purpose

Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:

Insert

BSD

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307-DPP4-blast

Plasmid

#158452

Purpose

Constitutive lentiviral expression of human DPP4.

Depositor

Alejandro Chavez, Sho Iketani

Insert

DPP4 (DPP4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast shGFP

Plasmid

#110419

Purpose

shGFP control, silence GFP gene, blasticidin selection.

Depositor

Sandra Martha Gomes Dias

Insert

GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 (RNA PolIII)

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRi(v1)-Blast

Plasmid

#170067

Purpose

Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

KRAB-dCas9

Use

CRISPR and Lentiviral

Tags

2A and 2xHA tag

Expression

Mammalian

Promoter

EFS-NS

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

Plasmid

#219565

Purpose

3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

Depositor

Andrea Ventura

Article

Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

Insert

HygroR_2A_H2B-GFP-N_loxP_mScarlet

Use

Unspecified

Promoter

EF-1a

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-ultraID-Blast

Plasmid

#207715

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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lentiBE3-SpRY-Blast

Plasmid

#199303

Purpose

Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

FNLS-BE3-SpRY

Use

CRISPR and Lentiviral

Tags

2A tag

Expression

Mammalian

Promoter

EFS

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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hMLKL-Venus-Plasma

Plasmid

#106081

Purpose

expression in mammalian cells

Depositor

Douglas Green

Article

Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

Insert

hMLKL-Venus-Plasma (MLKL Human)

Use

Retroviral; Tet-on retrovirus

Tags

plasma membrane-targetting domain of H-Ras (aa 1-…

Expression

Mammalian

Promoter

dox-inducible

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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Butelase AY

Plasmid

#221847

Purpose

Expresses Butelase AY with Val237Ala and Thr238Tyr mutations

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2024 Nov 30;23(1):325.

Insert

Butelase 1 ligase

Expression

Bacterial

Mutation

Val237Ala, Thr238Tyr

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

Plasmid

#211471

Purpose

lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

Depositor

James Eshleman

Article

Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

Insert

Cas9_T2A_mNeonGreen_P2A_BlasticidinR

Use

CRISPR and Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

hU6, EF-1a

Available Since

March 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_dCas9-KRAB-MeCP2_Blast

Plasmid

#221501

Purpose

Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

dCas9-KRAB-MeCP2-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-GshF-blasticidin

Plasmid

#210313

Purpose

Expresses humanized Streptococcus thermophilus GshF in mammalian cells

Depositor

Kivanc Birsoy

Article

Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

Insert

Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)

Use

Retroviral

Tags

FLAG peptide

Mutation

Codon optimized for expression in human cells

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX307-APN-blast

Plasmid

#158455

Purpose

Constitutive lentiviral expression of human APN.

Depositor

Alejandro Chavez, Sho Iketani

Insert

APN (ANPEP Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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LentiAsCpf1-3xMYC-blast

Plasmid

#127750

Purpose

Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal

Depositor

Giulio Draetta

Article

Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

Insert

AsCpf1

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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TPI in pCW57_tTA_Blast

Plasmid

#154209

Purpose

Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Tags

None

Expression

Mammalian

Promoter

TRE repeats (dox off)

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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mEmerald-Plastin-C-10

Plasmid

#54232

Purpose

Localization: Fimbrin/Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Plastin (PLS1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
qTAG-C-mStayGold-Blast

Plasmid

#227254

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2

Plasmid

#124903

Purpose

Lentiviral vector to express p65-mRuby2 under p65 promoter (With blasticidin resistance)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

Insert

p65 promoter- p65 CDS-mRuby2

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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BPK1520_blastR

Plasmid

#175289

Purpose

Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection

Depositor

Ronald Cohn

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV/U6

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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