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mHDAC7-Nterm (23-504) pEF6-V5

Plasmid

#73270

Purpose

Expression of mouse HDAC7-Nterm in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

His/V5

Expression

Mammalian

Mutation

deleted amino acids 505-938

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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NET23 pEGFP-N2 (1174)

Plasmid

#62037

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

NET23 (STING1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
mHDAC7-Unspliced (23-938) pEF6-Flag (MJS_018)

Plasmid

#73269

Purpose

mHDAC7-U (23-938) pEF6-V5 (MJS_017) (Flag tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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XE130 beta2AR Fz2-Cs2+

Plasmid

#16794

Depositor

Randall Moon

Article

Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

Insert

beta 2 AR Fz2 (Fzd1, Adrb2 Rat)

Use

Xenopus expression

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pSico shMAP3K2 mouse

Plasmid

#85657

Purpose

expression of shRNA targeting mouse MAP3K2

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

shRNA targeting 3'UTR of Map3k2 (Map3k2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSico shMAP3K2 human

Plasmid

#85656

Purpose

expression of shRNA targeting human MAP3K2

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

shRNA targeting 3'UTR of MAP3K2 (MAP3K2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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mEmerald-Vinculin-N-21

Plasmid

#54304

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mCherry-Vinculin-N-21

Plasmid

#55160

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pT7-RiboJ-LacZa-T7

Plasmid

#173224

Purpose

pFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systems

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pT7-RiboJ-mTFP1-T7

Plasmid

#173225

Purpose

Encodes mTFP fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mTFP1

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFGC-T7RibJ-RRvT

Plasmid

#173226

Purpose

Encodes RRvT fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

RRvT

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pGL4.23-GW

Plasmid

#60323

Purpose

This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Type

Empty backbone

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro-Scrambled

Plasmid

#47541

Purpose

Tet inducible scrambled shRNA.

Depositor

Charles Rudin

Article

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

Scrambled shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-YapS127A

Plasmid

#46049

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

Flag tag

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]

Plasmid

#84446

Purpose

AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Has Service

AAV1, AAV5, and AAV8

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

CAG

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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sgTP53(A)

Plasmid

#85559

Purpose

Expresses sgRNA targeting human TP53 around codon F109

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

tumor protein p53 (TP53 Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

Plasmid

#85798

Purpose

donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Mutation

homology arms for knock-in into first intron

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]

Plasmid

#78174

Purpose

AAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro-SOX2

Plasmid

#47540

Purpose

Tet inducible knockdown of SOX2.

Depositor

Charles Rudin

Article

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

SOX2 shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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