We narrowed to 13,608 results for: crispr cas9

1. pAAV-CMV-Hsp1Cas9

   Plasmid

   #192125

   Purpose

   Expresses Hsp1Cas9

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Hsp1Cas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX459-HypaCas9-CfANLN_sgRNA

   Plasmid

   #183880

   Purpose

   pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   Canis familiaris ANLN sgRNA spacer
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-TAC-KRAB-dCas9

   Plasmid

   #120355

   Purpose

   piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   tet-on and CBh

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. BII-ChPtW-eSpCas9

   Plasmid

   #133381

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX-TRE-dCas9-KRAB-MeCP2-BSD

   Plasmid

   #140690

   Purpose

   Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   Cas9m4-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

   Plasmid

   #108871

   Purpose

   Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

   Depositor

   Alex Schier

   Article

   Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

   Inserts

   5xU6:sgRNA
   Cas9-t2A-GFP
   

   Use

   CRISPR

   Promoter

   U6 and hsp70

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

   Plasmid

   #162334

   Purpose

   Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSaCas9-KRAB
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

   Plasmid

   #85452

   Purpose

   Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

   Depositor

   Aviv Regev

   Article

   Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

   Insert

   U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

   Plasmid

   #223127

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

    Plasmid

    #223113

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

    Plasmid

    #223114

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

    Plasmid

    #223115

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

    Plasmid

    #223116

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-TadCBEd-SpCas9(MRKQKC)-P2A-EGFP (AHK574)

    Plasmid

    #223134

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKQKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(MRKQKC)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/MRKQKC)=D1135M, …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-TadCBEd-SpCas9(MRKSKC)-P2A-EGFP (AHK446)

    Plasmid

    #223135

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MRKSKC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(MRKSKC)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/MRKSKC)=D1135M, …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-TadCBEd-SpCas9(KWRQLC)-P2A-EGFP (BKS1026)

    Plasmid

    #223126

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(KWRQLC)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/KWRQLC)=D1135K, …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-TadCBEd-SpCas9(LWRYEK)-P2A-EGFP (BKS1022)

    Plasmid

    #223128

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(LWRYEK)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/LWRYEK)=D1135L, …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-TadCBEd-SpCas9(MKRCMV)-P2A-EGFP (BKS1032)

    Plasmid

    #223129

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(MKRCMV)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/MKRCMV)=D1135M, …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-TadCBEd-SpCas9(MKRCKS)-P2A-EGFP (RAS3445)

    Plasmid

    #223130

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(MKRCKS)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/MKRCKS)=D1135M, …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-TadCBEd-SpCas9(ARGIMR)-P2A-EGFP (BKS1036)

    Plasmid

    #223132

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(ARGIMR)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/ARGIMR)=D1135A, …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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