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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,513 results for: emb

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Showing: 2361 - 2380 of 20513 results

  5. pTCF156 HLA-A*02:01

    Plasmid
    #180456
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180456-plasmid-map-sequence-id-355799

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  14. GFP-KIF1B beta-FL

    Plasmid
    #79108
    Purpose
    Mammalian expression of GFP-KIF1B beta-FL
    Depositor
    Susanne Schlisio
    Publication
    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFP-KIF1B beta full length (KIF1B Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    exon 25 missing (natural splice varient)
    Promoter
    CMV
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Publication
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117686-plasmid-map-sequence-id-236350

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  18. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid
    #59987
    Purpose
    Eef1a (EF1alpha) promoter driving nls::Cas9::nls
    Depositor
    Lionel Christiaen
    Publication
    Stolfi et al Development. 2014 Nov;141(21):4115-20. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nls::Cas9::nls
    Use
    CRISPR
    Mutation
    Reverted mutations from dCas9 to wiltdtype
    Promoter
    Ciinte.Eef1a (EF1alpha) -1955/-1
    Available since
    Nov. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59987-plasmid-map-sequence-id-95666

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Showing: 2361 - 2380 of 20513 results