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Plasmid#140984PurposeEncodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlpha15-RLuc8 (GNA15 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlpha13-RLuc8
Plasmid#140986PurposeEncodes a G alpha subunit (GNA13) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlpha13-RLuc8 (GNA13 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dCas9-FOG1[N+C]
Plasmid#100085PurposedCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFOG1(aa 1-45) (ZFPM1 Human)
UseCRISPRTags3XFLag-NLS-FOG1-dCas9-FOG1-NLSExpressionMammalianPromoterCMVAvailable sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-GFP-HYGRO
Plasmid#115144PurposeSafe harbor site 231 knock-in vector with GFP-HygromycinR expression cassetteDepositorInsertGFP
ExpressionMammalianPromoterEF1aAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEJS887_pTetR-P2A-BFPnls/sgAlpha
Plasmid#108651PurposeAlpha satellite repeats targeting Spy sgRNA under U6 promoterDepositorInsertsAlpha satellite repeats targeting sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianPromoterU6 and hPGKAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSMART-SOS-GFPuv
Plasmid#102283PurposeFluorescent sensor of E. coli SOS responseDepositorInsertcda-GFPuv
ExpressionBacterialPromotercda SOS promoterAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a_T5-ARG1
Plasmid#106476PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917DepositorInsertARG1
UseSynthetic BiologyExpressionBacterialPromoterT5Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPS22 (pET23a-Cas10/Csm/csm2H6N)
Plasmid#70038PurposeBacterial expression of the S. epidermidis Cas10·Csm complexDepositorInsertCas10/Csm complex
Tags6xHIS on N terminus of Csm2ExpressionBacterialPromoterT7Available sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-Cre
Plasmid#198712PurposeFor lentiviral expression of Cre recombinase in mammalian cells.DepositorInsertPgk-Cre
UseCre/Lox and LentiviralExpressionMammalianPromoterPGKAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
Plasmid#221235PurposeTet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.DepositorInsertTadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
UseLentiviralAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_r5-HT1.0
Plasmid#208719PurposeExpresses the red 5-HT sensor GRAB_r5-HT1.0 in neuronsDepositorInsertRed fluorescent 5-HT sensor GRAB_r5-HT1.0
UseAAVPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG1160 mCherry-FIREtag
Plasmid#205164PurposeExpresses mCherry -FIREtag in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-FIREtag
ExpressionMammalianAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion T2A-H2B-miRFP703
Plasmid#113097PurposeGeneral donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertT2A-H2B-miRFP703
UseSynthetic BiologyAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-RILP
Plasmid#74600Purposeused for expression in E. coli and purificationDepositorInsertRab-interacting lysosomal protein (RILP Human)
TagsFLAG, StrepII, and superfolder GFPExpressionBacterialPromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-yeastSMASh-YFP
Plasmid#68854PurposeExpresses SMASh tag fused to YFP at N terminus in yeastDepositorInsertyeastSMASh-YFP
TagsSMASh tagExpressionYeastPromoterGPDAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-Rab7a-7
Plasmid#55389PurposeLocalization: Ras GTPase, Excitation: 433, Emission: 475DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab7a (RAB7A Human)
TagsmCeruleanExpressionMammalianPromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Moesin-C-14
Plasmid#55103PurposeLocalization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMoesin (MSN Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS-PHFF-sh-hBVRA
Plasmid#100285PurposeExpression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cellsDepositorInsertsMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for human BVRA
TagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoter and H1 promoterAvailable sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-Ubiquitin-K11
Plasmid#121152PurposeMammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to argininesDepositorAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ecAT3.10
Plasmid#107215PurposeMammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertecAT3.10
ExpressionMammalianPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by