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Plasmid#205031PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2
UseTagsKKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H3
Plasmid#205032PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H3
UseTagsKKRRRKKKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCVD630
Plasmid#66163Purposecloning of ace geneDepositorInsertace
UseCloning vectorTagsExpressionMutationPromoterUnknownAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-30)
Plasmid#199167Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(-30)
UseTagsMGHHHHHHGGAExpressionMutationT19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+12)
Plasmid#199161Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+12)
UseTagsMGHHHHHHGGAExpressionMutationE16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+18)
Plasmid#199162Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+18)
UseTagsMGHHHHHHGGAExpressionMutationE16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSELt_S7.A
Plasmid#125835PurposeNegative selection plasmid for evolution of an amber stop codon suppression tRNADepositorInsertACP-2xAmb-pheS
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpr.trpL*Available sinceMay 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNoT7
Plasmid#218168PurposepET31b plasmid with short insert lacking T7 and ribosome binding sites as controlDepositorInsertShort sequence, mutated RBS and T7 sites
UseTagsExpressionBacterialMutationPromoterNA - mutated T7Available sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+6)-L1
Plasmid#205039PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(+6)-L1
UseTagsGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationE16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…PromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+6)-L2
Plasmid#205040PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(+6)-L2
UseTagsGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationE16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…PromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+6)-L3
Plasmid#205041PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(+6)-L3
UseTagsGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationE16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…PromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+6)-H1
Plasmid#205042PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(+6)-H1
UseTagsKRRRKK and MGHHHHHGGAExpressionBacterialMutationE16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…PromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+6)-H2
Plasmid#205043PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(+6)-H2
UseTagsKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationE16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…PromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+6)-H3
Plasmid#205044PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(+6)-H3
UseTagsKRRRKKKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationE16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…PromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)
Plasmid#205049PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)
UseTagsMGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)-L1
Plasmid#205033PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-L1
UseTagsGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)-L2
Plasmid#205034PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-L2
UseTagsGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)-L3
Plasmid#205035PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-L3
UseTagsGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)-H1
Plasmid#205036PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-H1
UseTagsKRRRKK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)-H2
Plasmid#205037PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-H2
UseTagsKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)-H3
Plasmid#205038PurposeFluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coliDepositorInsertGFP(0)-H3
UseTagsKRRRKKKRRRKKKRRRKK and MGHHHHHGGAExpressionBacterialMutationE16R, I138R, D207R, N222K, L246RPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecBCD GamS
Bacterial Strain#176586PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.DepositorBacterial ResistanceChloramphenicol and ampicillinAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BL21 Rosetta2 ΔrecB GamS
Bacterial Strain#176585PurposeE. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 + pBADmod1-linker2-gamS plasmids.DepositorBacterial ResistanceChloramphenicol and ampicillinAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-SP6
Plasmid#102418PurposeAc transposase with SP6 promoter for in vitro transcriptionDepositorInsertAc transposase
UseTagsExpressionBacterialMutationPromoterSP6Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+30)
Plasmid#199164Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+30)
UseTagsMGHHHHHHGGAExpressionMutationE16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+24)
Plasmid#199163Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+24)
UseTagsMGHHHHHHGGAExpressionMutationE16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L1
Plasmid#205050PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L1
UseTagsGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L2
Plasmid#205051PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L2
UseTagsGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-6)-H2_mCherry(-6)-L3
Plasmid#205052PurposeCo-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coliDepositorInsertGFP(-6)-H2_mCherry(-6)-L3
UseTagsGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGAExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+36)
Plasmid#199165Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+36)
UseTagsMGHHHHHHGGAExpressionMutationE16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQnl_Pcphy23
Plasmid#191354PurposeClostridium plasmid expressing NanoLuc reporter from moderate, constitutive promoterDepositorInsertPcphy23-NanoLuc
UseTagsExpressionBacterialMutationPromoterPcphy23Available sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod3C-GG
Plasmid#191350PurposeClostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Arf6 T27N- Venus in pcDNA3.1
Plasmid#90464PurposeRhoA mediated cell migrationDepositorInsertArf6 T27N (ARF6 )
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQmod2C-GG
Plasmid#191347PurposeClostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATmin-GG
Plasmid#191352PurposeClostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQdC12a
Plasmid#191356PurposeClostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expressionDepositorInsertsPGusA2-TetO2/1-dCas12a (LbCpf1)
miniPthl-tetR
UseTagsExpressionBacterialMutationPromoterPGus2A-TetO2/1 and miniPthlAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod2E-GG
Plasmid#191345PurposeClostridium expression vector (pBP1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod3E-GG
Plasmid#191348PurposeClostridium expression vector (pCB102 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod4E-GG
Plasmid#191351PurposeClostridium expression vector (pCD6 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only