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Showing: 441 - 480 of 700 results

  4. PB-CT3G-CERP2-MG-IRES2-mCherry

    Plasmid
    #175507
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175507-plasmid-map-sequence-id-345876

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  5. PB-CT3G-CERP2-MG-IRES2-mTagBFP2

    Plasmid
    #175509
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mTagBFP2 (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175509-plasmid-map-sequence-id-345099

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  13. pET21-pelB-LaG-6

    Plasmid
    #172741
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-6
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172741-plasmid-map-sequence-id-340175

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  20. pMCSG28-SH3-588

    Plasmid
    #185920
    Purpose
    Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase
    Depositor
    Brian Kuhlman
    Article
    Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d
    Insert
    SH3-588
    Use
    Tags
    6x HIS tag
    Expression
    Bacterial
    Mutation
    Designed enzyme with novel interface between huma…
    Promoter
    T7
    Available since
    Dec. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    185920-plasmid-map-sequence-id-369048

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  21. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

    Plasmid
    #175506
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    175506-plasmid-map-sequence-id-345872

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  22. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid
    #58711
    Purpose
    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
    Depositor
    Hermann Gaub
    Article
    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm
    Insert
    Xyl-DocI
    Use
    Tags
    HIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    changed Threonin 120 to Cysteine in Xylanase Doma…
    Promoter
    T7
    Available since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58711-plasmid-map-sequence-id-90776

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  24. JAB3312

    Plasmid
    #190406
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt)
    Depositor
    Jose Dinneny, Jennifer Brophy
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-PhlFO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    190406-plasmid-map-sequence-id-391159

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  25. JAB3494

    Plasmid
    #190418
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter )
    Depositor
    Jose Dinneny, Jennifer Brophy
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_<<2XAtMAR10>> 6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    190418-plasmid-map-sequence-id-393672

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  26. JAB2602

    Plasmid
    #190389
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt)
    Depositor
    Jose Dinneny, Jennifer Brophy
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_4XAmtRO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    190389-plasmid-map-sequence-id-393424

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  31. JAB2603

    Plasmid
    #190390
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)
    Depositor
    Jose Dinneny, Jennifer Brophy
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    190390-plasmid-map-sequence-id-393426

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  36. pET21-pelB-LaG-9

    Plasmid
    #172742
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-9
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172742-plasmid-map-sequence-id-340182

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  37. attB-P7-attP-YFP

    Plasmid
    #195571
    Purpose
    A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.
    Depositor
    Richard Murray
    Article
    Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52
    Insert
    Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    P7
    Available since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    195571-plasmid-map-sequence-id-383991

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  38. pET21-pelB-LaG-8

    Plasmid
    #172761
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-8
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172761-plasmid-map-sequence-id-340116

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  39. pET21-pelB-LaG-5

    Plasmid
    #172760
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-5
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172760-plasmid-map-sequence-id-340022

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  40. pET21-pelB-LaG-2

    Plasmid
    #172739
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-2
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172739-plasmid-map-sequence-id-340192

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Showing: 441 - 480 of 700 results