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Plasmid#126986PurposeLevel 2 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only
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pJUMP22x-1A(sfGFP)
Plasmid#126969PurposeLevel 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL1_35
Plasmid#199042PurposeLevel 1 plasmid, barcoded ORIDepositorInsertRK2 ORI plus NGS barcode 12
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_166
Plasmid#199098PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertRK2 ORI plus NGS barcode 12, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNDGG003
Plasmid#231316PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNuc-cis
Plasmid#202796PurposeRK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killingDepositorInsertTevSpCas9, sgRNA cassette, oriT, CenArsHis
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNDGG054
Plasmid#231323PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG050
Plasmid#231319PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG010, pOGG012, and pOGG014, GmRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAT.011
Plasmid#119555PurposeSelf replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX-Z4 (GB3375)
Plasmid#160648PurposepLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-Tpr
Plasmid#60882PurposeExpresses Flag-Tagged nucleoporin Tpr in Mammalian CellsDepositorInsertTpr (TPR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB-EPCP5
Plasmid#228879PurposeExpression of Z-tagged LRRK2 KW using BaculovirusesDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
UseTagsHis-Z-TEVExpressionInsectMutationAmino acids deleted 1-1846PromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFB-EPCP1
Plasmid#226783PurposeExpression of Z-tagged LRRK2 RCKW using BaculovirusesDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
UseTagsHis-Z-TEVExpressionInsectMutationAmino acids deleted 1-1326PromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGE2.0
Plasmid#165984PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.DepositorInsertsRK2/RP4 origin of transfer
repA2B2C2
nourseothricin N-acetyl transferase
tetracycline resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…TagsExpressionMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBGE2.0
Plasmid#165987PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.DepositorInsertsRK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
tetracycline resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…TagsExpressionMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEVS104
Plasmid#207393PurposeConjugal helper plasmidDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-3xHA-KCNJ6-mCherry
Plasmid#197032PurposeExpress 3xHA-tagged human KCNJ6 (Girk2), co-expressed with mCherry, from UbiC promoterDepositorInsertKCNJ6 (KCNJ6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCWXPGR-pTF-ERK:MEK
Plasmid#114291PurposeTET-inducible expression of ERK:MEK fusion proteinDepositorInsertrERK2 and hMEK1 proteins (Mapk1 Rat, Human)
UseLentiviralTagsExpressionMutationrat ERK2 fused to human MEK1 with a linker in bet…PromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH7m34GW-35S:SNACS
Plasmid#203576PurposePlant SnRK2 kinase activity FRET sensor in plant expression vectorDepositorInsertSNACS
UseTagsStrepIIExpressionPlantMutationnonePromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH7m34GW-35S:SNACS S785A
Plasmid#203577PurposePlant SnRK2S30A kinase activity FRET sensor in plant expression vectorDepositorInsertSNACS S785A
UseTagsStrepIIExpressionPlantMutationS785APromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-Parkin
Plasmid#23956PurposeMammalian expression of human Park2 fused to mCherryDepositorInsertPark2 (PRKN Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
YFP-Parkin
Plasmid#23955PurposeMammalian expression of human Park2 fused to EYFPDepositorInsertPark2 (PRKN Human)
UseTagseYFPExpressionMammalianMutationPromoterAvailable sinceApril 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
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HA-TAZ-4D
Plasmid#229416PurposeLentiviral or overpexression of cDNADepositorInsertTAZ-4D (WWTR1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S58,S60,S62,S90) mutated to DPromoterEFSAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-TAZ-4A
Plasmid#229414PurposeLentiviral or overpexression of cDNADepositorInsertTAZ-4A (WWTR1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S58,S60,S62,S90) mutated to APromoterEFSAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MST2-S444A
Plasmid#229420PurposeLentiviral or overpexression of cDNADepositorInsertMST2-S444A (STK3 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosite mutant (S444A)PromoterEFSAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR SFFVp BFP-Erk D319N
Plasmid#102350PurposeLentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutationDepositorInsertErk2 (Mapk1 Mouse)
UseLentiviralTagsBFPExpressionMutationAspartic acid 319 was mutated to asparaginePromoterSFFVAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-MST1-7A
Plasmid#229418PurposeLentiviral or overpexression of cDNADepositorInsertMST1-7A (MST1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (T175,T273,S320,S327,T340,T387…PromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-YAP-5D
Plasmid#229415PurposeLentiviral or overpexression of cDNADepositorInsertYAP-5D (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S128,S163,S350,T354,T398) mut…PromoterEFSAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-YAP-5A
Plasmid#229413PurposeLentiviral or overpexression of cDNADepositorInsertYAP-5A (YAP1 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S128,S163,S350,T354,T398) mut…PromoterEFSAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MAP4K4-10A
Plasmid#229403PurposeLentiviral or overpexression of cDNADepositorInsertMAP4K4 (MAP4K4 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S523,S536,S547,S643,T684,S726…PromoterEFSAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MAP4K4-10D
Plasmid#229404PurposeLentiviral or overpexression of cDNADepositorInsertMAP4K4 (MAP4K4 Human)
UseLentiviralTagsHAExpressionMammalianMutationMARK2 phosphosites (S523,S536,S547,S643,T684,S726…PromoterEFSAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE-Truncated-Kir3.2-StreptagII
Plasmid#177263PurposeExpresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cellsDepositorInsertG-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
UseTagsStreptagIIExpressionInsectMutationResidues 49-378Promoterpolyhedrin promoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRaf-IRES-pErk reporter
Plasmid#226170PurposeUsed to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.DepositorUseTags3xFlag-tdMCP and MycExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBGE3.0
Plasmid#165988PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.DepositorInsertsRK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
neomycin resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…TagsExpressionMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBGE1.0
Plasmid#165986PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Spec resistance.DepositorInsertsRK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
spectinomycin/streptomycin resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…TagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only