Addgene

We narrowed to 2,785 results for: 2-Oct

Showing: 221 - 240 of 2785 results

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pCDNA3-SREBP-2-GFP (A1134-1135)

Plasmid

#204203

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Use

Tags

T7x3 and eGFP

Expression

Mammalian

Mutation

Gly to Ala 1134, 1135

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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Mouse genome-wide lentiviral CRISPR gRNA library version 2

Pooled Library

#67988

Purpose

Mouse genome-wide CRISPR guide RNA library V2. (pKLV2-U6gRNA5(MouseV2)-PGKpuro2ABFP-W)

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Species

Homo sapiens, Mus musculus, and Synthetic

Use

CRISPR and Lentiviral

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag beta-2-adrenergic-receptor

Plasmid

#14697

Depositor

Robert Lefkowitz

Article

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B2AR (ADRB2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO R99A CoV-2 nsp1 3xFLAG

Plasmid

#176061

Purpose

Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mBltp1 (KIAA1109) - 2

Plasmid

#198490

Purpose

lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mBltp1 (4932438A13Rik Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-GCaMP6f-Fishell-2

Plasmid

#83899

Purpose

GCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-AVI

Plasmid

#160475

Purpose

Expresses SARS-CoV-2 NTD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-NTD

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-AVI

Plasmid

#160476

Purpose

Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-RBD

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-AVI

Plasmid

#160474

Purpose

Expresses SARS-CoV-2 S2P protein with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

Insert

SARS-CoV-2-S2P

Use

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

Plasmid

#84250

Purpose

Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgTRE3G

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-bio-myc-mCE 2-210

Plasmid

#112715

Purpose

Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Use

Tags

biotinylation signal peptide (Tagwerker et al. 20…

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shFDPS #2

Plasmid

#198760

Purpose

conditional knockdown of FDPS

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

Insert

shFDPS #2 (FDPS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pSG5HA-MAGE-2-429

Plasmid

#89133

Purpose

mammalian expression of N-terminal HA-tagged full-length human MAGE-A11 amino acid residues 2-429

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2009 Dec 11;284(50):34793-8

Insert

MAGE-A11 (MAGEA11 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

contains aa 2-429

Promoter

SV40

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

Plasmid

#172984

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-KRAB_sgSTK4i_#2 (PuroR)

Plasmid

#209774

Purpose

CRISPRi for STK4

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

dCas9-KRAB, sgSTK4i (STK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-His-TEV-A7-Strep (2-180)

Plasmid

#190067

Purpose

Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

Depositor

Lalit Deshmukh

Article

Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

Insert

Annexin A7 PRD (ANXA7 Human)

Use

Tags

GB1-His-TEV and Strep

Expression

Bacterial

Mutation

PRD only, residues 2-180

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCG

Plasmid

#51476

Purpose

mammalian cell expression vector

Depositor

Winship Herr

Article

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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pSrpR-BCD7-tetR-ssrA P65U18C45T14 v40-2 (491)

Plasmid

#70736

Purpose

plasmid for building 3n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-BCD7-lambdaCI-ssrA P65U18C47T14 v40-2 (495)

Plasmid

#70735

Purpose

plasmid for building 5n1

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

lambdaCI-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-BCD7-betI-ssrA P65U18C64T14 v50-2 (567)

Plasmid

#70734

Purpose

plasmid for building 3n1, 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

betI-ssrA

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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