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Plasmid#22865DepositorInsertK27 HMR
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X
Plasmid#147924PurposeMammalian Expression of 6xbsMS2-MalatDepositorInsert6xbsMS2-Malat (Malat1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template1-3XMyc-Spacer
Plasmid#176208PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template1-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2 Lats1-PY1+2 mutant
Plasmid#18975DepositorInsertLarge tumor suppressor kinase 1 PY1+2 mutant (LATS1 Human)
UseTags2xFlagExpressionMammalianMutationPY1 mutant, (Tyrosine 376 changed to Alanine) and…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template3-3XMyc-Spacer
Plasmid#176210PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template3-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template2-3XMyc-Spacer
Plasmid#176209PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template2-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2 Lats1-PY1 mutant
Plasmid#18973DepositorInsertLarge tumor suppressor kinase 1 PY-1 mutant (LATS1 Human)
UseTags2xFlagExpressionMammalianMutationPY1 mutant, Tyrosine 376 changed to AlaninePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-S28 histone 3 phosphorylation reporter
Plasmid#32788DepositorInsertCFP-14-3-3tau-H3 peptide-YFP
UseTagsCFP and YFPExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2 Lats1-PY2 mutant
Plasmid#18974DepositorInsertNM_004690 (LATS1 Human)
UseTags2xFlagExpressionMammalianMutationPY2 mutant (Tyrosine 559 changed to Alanine)PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
human SLC12A5-V5-HA-2A-Luciferase repair template
Plasmid#158576PurposeFor targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.DepositorInserthuman SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
UseTagsV5 and HA on the endogenous KCC2 protein productExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
AtS/MAR10 insulator sequence (GB3458)
Plasmid#160638PurposepEGB3a1 AtS/MAR10DepositorInsertAtS/MAR10 insulator sequence
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-mutated V1 O-Glycosylation site
Plasmid#120406Purposeenables eukaryotic expression of human plasma fibronectin in which the third O-glycosylation site was mutated from threonine to serineDepositorInsertFibronectin (FN1 Human)
UseTagsExpressionMammalianMutationContains complete variable region with a mutation…PromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR4-TOPO_L31-FLAG-TEV-AviTag_repair_template
Plasmid#235608PurposeRepair template for inserting FLAG-TEV protease cleavage site-AviTag into the C terminus of mouse Rpl31 via CRISPRDepositorInsertRpl31 C-terminus with FLAG-TEV-AviTag and flanking homology arms
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
Poliovirus 1 PV1 isolate 09_05_19 3C protease
Plasmid#228647PurposeBacterial expression of the Polio 3C proteaseDepositorInsertPolio virus 3C protease
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
SARS-CoV-2 isolate:Wuhan-Hu-1 nsp3 macrodomain
Plasmid#228643PurposeBacterial expression of the SARS-CoV-2 nsp3 macrodomain for crystallization of SARS-CoV-2 nsp3 macrodomain in P 4 3DepositorInsertSARS-CoV-2 NSP3 macrodomain (ORF1ab SARS-CoV-2)
UseTagsHis-TevExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Coxsackievirus B3 (CVB3) 3C protease isolate KR-2002
Plasmid#228639PurposeBacterial expression of the Coxsackievirus 33 isoform B 3C proteaseDepositorInsertCoxsackievirus 33 isoform B 3C protease
UseTagsHis-SUMOExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
T7-LacO-Halo-PILATeS-RGD (200nM)
Plasmid#216716PurposeExpresses HaloTag-PILATeS-RGD with 200 nM BiT tag in bacteriaDepositorInsertHaloTag-PILATeS-RGD
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7-LacO-Halo-PILATeS-RGD (700pM)
Plasmid#216714PurposeExpresses HaloTag-PILATeS-RGD with 700 pM BiT tag in bacteriaDepositorInsertHaloTag-PILATeS-RGD
UseTags6xHis and HaloTagExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-PlatTET-wpA-pPuroTK
Plasmid#214125PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertdCas9-TET
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH
Plasmid#148878PurposeMammalian Expression of TNFalpha-ARE-polyA-MalatDepositorInsertTNFalpha-ARE-polyA-Malat (TNF Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only