We narrowed to 579 results for: SPY
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Plasmid#231576PurposePotent light-deactivated CRISPRaDepositorInsertdSpyCas9-VPR with an AsLOV2 insertion behind E566
UseCRISPRExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD28(CD45TM)
Plasmid#223624PurposeLentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cellsDepositorInsertCD28 (CD28 Human)
UseLentiviral; mammalian expressionTagsSpytag003MutationCD28 transmembrane domain replaced by CD45 transm…Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Spy-CD28(Y191F)
Plasmid#223623PurposeLentiviral expression of human CD28 (Y191F) with SpyTag003 in mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTag-SpyTag-(AffiHER2)x3
Plasmid#216281PurposeThree affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate CatcherDepositorInsertSnoopTag-SpyTag-(AffiHER2)x3
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, and SpyTagExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-RBD Omicron BQ.1.1
Plasmid#214725PurposeExpresses SARS-CoV-2 Omicron BQ.1.1 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cellsDepositorAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher (Min)
Plasmid#216713PurposeExpresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014DepositorInsertCys-SpyCatcher (Min)
Tags6xHisExpressionBacterialMutationSee Li...Howarth, J. Mol. Biol., 2014Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZa pLtetO CsgA-SpyTag
Plasmid#204503PurposeBacterial expression of CsgA with C-terminal SpyTag peptide and GS linkerDepositorInsertCsgA
UseSynthetic BiologyTags6xHis, SpyTagExpressionBacterialPromoterpLtetOAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFN-HaloTag-TalinR7R8-SpyTag
Plasmid#192046PurposePlasmid expresses human talin R7R8 domain flanked between a HaloTag and a SpyTagDepositorAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-hcvs-SpyCatcher
Plasmid#179677PurposeHCVP-cleavable ER-associated SpyCatcherDepositorInsertHCVP SpyCatcher
ExpressionMammalianAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop B
Plasmid#171777PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-SpyTag003 Loop B
TagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop C
Plasmid#171778PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174DepositorInsertsfGFP-SpyTag003 Loop C
TagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-Coh-His-SpyTag
Plasmid#157673PurposeExpression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag.DepositorInsertCohesin (R.f) with Histag and SpyTag
TagsHistag and SpyTagExpressionBacterialPromoterT5Available SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
TagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-CL7-2NLS-SpyCas9(dS)-A22p3star
Plasmid#222970PurposeBacterial expression of A22p-fused SpyCas9 constructDepositorInsertHis-CL7-2NLS-SpyCas9(dS)-A22p3
UseCRISPRTagsCL7 and HisExpressionBacterialPromotertacAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpyTag-Flag-SRSF3-V5-mCherry
Plasmid#235091PurposeSRSF3 mCherry fusion protein without MCP domainDepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpyTag-Flag-SRSF1-V5-mCherry
Plasmid#235089PurposeSRSF1 mCherry fusion protein without MCP domainDepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-BX SpyCatcher003-(short)mCD80
Plasmid#210567PurposeExpresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domainDepositorInsertSpycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
UseLentiviralExpressionMammalianPromoterSFFVAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere
Plasmid#85715PurposeTelomere-targeting Spy sgRNA under U6 promoterDepositorInserttelomere sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SpyTag-Flag-Lbr-V5-mCherry
Plasmid#235094PurposeLbr mCherry fusion protein without MCP domainDepositorAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)
Plasmid#120293PurposeAAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffoldDepositorInsertN-terminal fragment of SpyCas9
UseAAV and CRISPRTagsSV40-NLS and split-inteinExpressionMammalianAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only