We narrowed to 7,152 results for: Cad
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Plasmid#214047PurposeBacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
ExpressionBacterialMutationK121E, Q122A, N274A, R276E, Y285A, F286APromoterlacUV5 promoterAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT_PCaspase-N-C-term
Plasmid#214046PurposeBacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
ExpressionBacterialMutationaa 1-153, 154-666PromoterlacUV5 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT-sm_PCaspase
Plasmid#214048PurposeBacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
ExpressionBacterialMutationR456E, D459K, D476K, R479EPromoterlacUV5 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-SAVED-CHAT
Plasmid#214030PurposeBacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceumDepositorInsertSAVED-CHAT
TagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-dSAVED-CHAT
Plasmid#214031PurposeBacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceumDepositorInsertSAVED-CHAT
TagsStrep-tag IIExpressionBacterialMutationH375A, C422APromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-SAVED-CHAT-sm
Plasmid#214033PurposeBacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceumDepositorInsertSAVED-CHAT
TagsStrep-tag IIExpressionBacterialMutationR456E, D459K, D476K, R479EPromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-PCaspase
Plasmid#214034PurposeBacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceumDepositorInsertPCaspase
TagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) T2C, C90A, T198C
Plasmid#220235Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
TagsHis6-GB1-tevExpressionBacterialMutationT2C, C90A, T198CPromoterT7Available SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) C90A, T172C, T259C
Plasmid#220234Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
TagsHis6-GB1-tevExpressionBacterialMutationC90A, T172C, T259CPromoterT7Available SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Hyg-CMV-BCL9
Plasmid#202667PurposeBCL9 overexpression vector with hygromycin resistanceDepositorAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A HA pMT2
Plasmid#206111Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin bindingDepositorAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV5D HA-Δ37, 115-133, 204-217 PPM1H
Plasmid#207344PurposeMammalian expression of HA-Δ37, 115-133, 204-217 PPM1HDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_494-546
Plasmid#196531PurposeEntry vector for Gateway with Eps8L2_494-546DepositorAvailable SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_367-496
Plasmid#196530PurposeEntry vector for Gateway with Eps8L2_367-496DepositorAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_1-183
Plasmid#196527PurposeEntry vector for Gateway with Eps8L2_1-183DepositorAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_544-715
Plasmid#196532PurposeEntry vector for Gateway with Eps8L2_544-715DepositorAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_181-299
Plasmid#196528PurposeEntry vector for Gateway with Eps8L2_181-299DepositorAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-Eps8L2_1-496
Plasmid#196516PurposeExpression of GST-Eps8L2_1-496DepositorInsertEps8L2_1-496 (EPS8L2 Human)
TagsGSTExpressionBacterialMutationSilent mutations for siRNA resistancePromoterlac promoterAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_1-183
Plasmid#196535PurposeExpression of GST-Eps8L2_1-183DepositorInsertEps8L2_1-183 (EPS8L2 Human)
TagsGSTExpressionBacterialPromoterT7 promoter; lac UV5 promoterAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_181-299
Plasmid#196536PurposeExpression of GST-Eps8L2_181-299DepositorInsertEps8L2_181-299 (EPS8L2 Human)
TagsGSTExpressionBacterialPromoterT7 promoter; lac UV5 promoterAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_544-715
Plasmid#196540PurposeExpression of GST-Eps8L2_544-715DepositorInsertEps8L2_544-715 (EPS8L2 Human)
TagsGSTExpressionBacterialPromoterT7 promoter; lac UV5 promoterAvailable SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nmyc-Eps8L2-FL-siR
Plasmid#196514PurposeExpression of Myc-Eps8L2-FLDepositorInsertEps8L2-FL-siR (EPS8L2 Human)
TagsMycExpressionMammalianMutationSilent mutations for siRNA resistancePromoterT7 promoterAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Eps8L2_529-715
Plasmid#196533PurposeEntry vector for Gateway with Eps8L2_529-715DepositorAvailable SinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_297-370
Plasmid#196537PurposeExpression of GST-Eps8L2_297-370DepositorInsertEps8L2_297-370 (EPS8L2 Human)
TagsGSTExpressionBacterialPromoterT7 promoter; lac UV5 promoterAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_367-496
Plasmid#196538PurposeExpression of GST-Eps8L2_367-496DepositorInsertEps8L2_367-496 (EPS8L2 Human)
TagsGSTExpressionBacterialPromoterT7 promoter; lac UV5 promoterAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Eps8L2_494-546
Plasmid#196539PurposeExpression of GST-Eps8L2_494-546DepositorInsertEps8L2_494-546 (EPS8L2 Human)
TagsGSTExpressionBacterialPromoterT7 promoter; lac UV5 promoterAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN687
Plasmid#177363PurposeExpresses human KIF1A(1-393)(P305L) fused with leucine zipper and His tagDepositorInsertKIF1A (KIF1A Human)
TagsHis tag and Leucine zipperExpressionBacterialMutationchanged Proline 305 to LeucinePromoterT7Available SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
mitoTALECD for_atp1_1397NC
Plasmid#186204PurposeFor genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87DepositorInsertsmitochondrial targeted TALE left hand with 1397N of Cytidine Deaminase (mitoTALECD)
mitochondrial targeted TALE right hand with 1397C of Cytidine Deaminase (mitoTALECD)
UseSynthetic Biology; Ti plasmid for plant transform…PromoterArabidopsis RPS5AAvailable SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
mitoTALECD for_-20G_1333CN
Plasmid#186200PurposeFor genome editing (base editing) of Arabidopsis mitochondria, targeted 20th G to A of RNA editing site of ATP1 by OTP87DepositorInsertsmitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
UseSynthetic Biology; Ti plasmid for plant transform…Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
mitoTALECD for_ -13G_1397CN
Plasmid#186199PurposeFor genome editing (base editing) of Arabidopsis mitochondria, targeted -13th G to A of RNA editing site of ATP1 by OTP87DepositorInsertsmitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
UseSynthetic Biology; Ti plasmid for plant transform…Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
mitoTALECD for_ -6G_1333CN
Plasmid#186198PurposeFor genome editing (base editing) of Arabidopsis mitochondria, targeted -6th G to A of RNA editing site of ATP1 by OTP87DepositorInsertsmitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
UseSynthetic Biology; Ti plasmid for plant transform…Available SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D
Plasmid#175051PurposeOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coliDepositorExpressionBacterialMutationchanged Thr76 to Asp, phosphomimetic mutationPromoterT7 promoterAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn1-L713F
Plasmid#173937PurposeBacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn1-L713F (PARP1 Human)
Tags6xHisExpressionBacterialMutationDeletion of residues 1-96; L713F, V762AAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA
Plasmid#175050PurposeOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coliDepositorExpressionBacterialPromoterT7 promoterAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G871L
Plasmid#174798PurposeBacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A870L
Plasmid#174797PurposeBacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-K893I
Plasmid#174799PurposeBacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)DepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-SAE1_sh3
Plasmid#168985PurposeExpresses RFP cDNA and SAE1 shRNA 3 in mammalian cellsDepositorAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCVhyg-HA-FLAG_Ubc9_C93A
Plasmid#164937PurposeExpresses FLAG & HA tagged human Ubiquitin Conjugating Enzyme 9 (UBC9) _C93A mutant cDNADepositorAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
QuasAr-P2A-hKvb1
Plasmid#154083PurposeIndependent expression of QuasAr and human Kvbeta 1 in pFCK vectorDepositorInsertQuasAr-P2A-human Kvb1 (KCNAB1 Synthetic, Human)
UseLentiviralTagsmyc-FlagExpressionMammalianPromoterCaMKIIAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-3HA-GK_v2
Plasmid#134277PurposeLentivector encoding 3XHA-tagged GK (isoform 2)DepositorInsertGk (Gk Mouse)
UseLentiviralTags3X HAExpressionMammalianMutation553 amino acids, isoform 2PromoterCMVAvailable SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDM304_YFP-IqgC_mRFP-Raf1(RBD)
Plasmid#128698Purposesimultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cellsDepositorInsertsiqgC (full length)
Raf1 kinase (aa 50-132)
UseDictyostelium discoideum expression vectorTagsYFP and mRFPMutationnoneAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pABD823
Plasmid#67911PurposeProtein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiaeDepositorInsertCold temperature germinating 10 (CTG10)
TagsGAL4 DNA binding domain (148 amino acids)ExpressionYeastPromoterpADH1 (alcohol dehydrogenase 1 promoter)Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pABD933
Plasmid#67917PurposeEntry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in plantaDepositorInsertCTG10
UseGateway cloning vectorAvailable SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hE2F7mg_6ntmut
Plasmid#73075Purposehuman E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding siteDepositorInsertE2F7 (E2F7 Human)
ExpressionMammalianMutationpartial gene, exons 11,12,13 spaced by introns wi…Available SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hE2F7mg_10ntmut
Plasmid#73076Purposehuman E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding siteDepositorInsertE2F7 (E2F7 Human)
ExpressionMammalianMutationpartial gene, exons 11,12,13 spaced by introns wi…Available SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
N295A (EcN), E166Q (EcN) MetNI LmH
Plasmid#118269PurposeN295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structureDepositorTags6x His TagExpressionBacterialMutationChanged Glutamic Acid (Glu) 166 to Glutamine (Gln…PromoterT7 PromoterAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Met1 diUb (1-152, R42W)
Plasmid#110767PurposeBacterial expression of Met1 diUbDepositorAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP1893-1
Plasmid#105248Purposehuman GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)DepositorInsertInsertion of GFP11 at cut tagRFP 33bp downstream (recoded *) in Lamin A/C (LMNA Human)
ExpressionBacterialAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Adeno BN
Plasmid#101823PurposeAdenovirus for the expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1DepositorUseAdenoviralTagsFLAG-Cas9Available SinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only