We narrowed to 116,650 results for: mpi
-
Plasmid#120974PurposeMoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.DepositorInsertdCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C61m
Plasmid#120977PurposeMoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.DepositorInsertRpaR from Scott and Hasty 2016
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C62m
Plasmid#120978PurposeMoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.DepositorInsertTraR(W) from Scott and Hasty 2016
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P12m
Plasmid#120938PurposeMoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pLasTetO
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P15m
Plasmid#120939PurposeMoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pCinTetO
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P16m
Plasmid#120940PurposeMoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pCinLacO m
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P32m
Plasmid#120943PurposeMoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.DepositorInsertPlacO (Tim Lu, Matt Bennett, etc)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P33m
Plasmid#120944PurposeMoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP11 from Mutalik library (medium)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P34m
Plasmid#120945PurposeMoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP13 from Mutalik library (weak)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RNF125 Ring Mutant
Plasmid#122046Purposeexpress RNF125 RMDepositorInsertRNF125 RM (RNF125 Human)
UseTagsFlagExpressionMammalianMutationRing mutant C37A, C40APromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
brr2-C520R pRS313
Plasmid#111412Purposebrr2-C520R (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationC520R aa mutationPromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
brr2-N1104A pRS313
Plasmid#111414Purposebrr2-N1104A (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationN1104A aa mutationPromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107L pRS313
Plasmid#111416Purposebrr2-R1107L (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationR1107L aa mutationPromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 TCERG1L WW domain #2
Plasmid#104281PurposeBacterial expression of WW domain #2 from TCERG1LDepositorInsertTCERG1L WW domain #2 (TCERG1L Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 STXBP4 WW domain
Plasmid#104265PurposeBacterial expression of WW domain from STXBP4DepositorInsertSTXBP4 WW domain (STXBP4 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 PIN1L WW domain
Plasmid#104269PurposeBacterial expression of WW domain from PIN1LDepositorInsertPIN1L WW domain (PIN1P1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
mr-GST N-term fusion
Plasmid#112983PurposeFor protein expression and purification of N-terminus of Drosophila mr (APC2)DepositorInsertmr (APC2) (mr Fly)
UseTagsExpressionBacterialMutationN-term onlyPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 SMURF1 WW domain #1
Plasmid#104237PurposeBacterial expression of WW domain #1 from SMURF1DepositorInsertSMURF1 WW domain #1 (SMURF1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 SMURF1 WW domain #2
Plasmid#104238PurposeBacterial expression of WW domain #2 from SMURF1DepositorInsertSMURF1 WW domain #2 (SMURF1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
DsRed-CPI-17-T38A-NES
Plasmid#100791PurposeMammalian expression of Fluorescent CPI-17 neutral mutation cytosolicDepositorInsertPPP1R14A (PPP1R14A Human)
UseTagsDsRedExpressionMammalianMutationT38APromoterCMVAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
5U9N (PP-BRD3)
Plasmid#101255PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-BRD3
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
371-R
Plasmid#97033PurposeStrepII-tev-yORFDepositorTypeEmpty backboneUseTagsStrepII tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
371-L
Plasmid#97031PurposeFLAG-tev-yORFDepositorTypeEmpty backboneUseTagsFLAG tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-HFSpCas9-H840A
Plasmid#80455Purposehigh fidelity SpCas9 mammalian expression vectorDepositorInsertHFSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-WFS1
Plasmid#79276PurposeMammalian expression of WFS1 with an N-terminal GSTDepositorInsertWFS1 (WFS1 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-SIRPT1-Xenopus (Silurana) tropicalis
Plasmid#75323PurposeGST fusion Xenopus (Silurana) tropicalis Sacsin 87-1376DepositorInsertSacsin Xenopus (Silurana) tropicalis 87-1376
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Ceratotherium-simum-simum
Plasmid#78111PurposeNt His-tag Ceratotherium-simum-simum Sacsin 89-1366DepositorInsertSacsin Ceratotherium-simum-simum 89-1366
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Mustela putorius furo
Plasmid#78116PurposeNt His-tag Mustela putorius furo Sacsin 94-1381DepositorInsertSacsin Mustela putorius furo 94-1381
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Ceratotherium simum simum
Plasmid#75331PurposeGST fusion Ceratotherium simum simum Sacsin 89-1366DepositorInsertSacsin Ceratotherium simum simum 89-1366
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Mustela putorius furo
Plasmid#75333PurposeGST fusion Mustela putorius furo Sacsin 94-1381DepositorInsertSacsin Mustela putorius furo 94-1381
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP81xV5_ccdB
Plasmid#79019PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selctionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pREP82xV5_ccdB
Plasmid#79020PurposeGateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selectionDepositorTypeEmpty backboneUseTagsdouble V5 epitope tagExpressionYeastMutationPromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse NCKX2-AS-short (pcDNA3.1+)
Plasmid#75198PurposeMammalian expression of SLC24A2DepositorInsertSLC24A2 (Slc24a2 Mouse)
UseTagsExpressionMammalianMutationalternatively spliced exon absentPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK241
Plasmid#71693PurposeProduces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp404>Phe mutant (AarAH6-W404F)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationchanged Tro404 to PhePromoterT7 promoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK699
Plasmid#71691PurposeProduces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Asp371>Asn mutant (AarAH6-D371N)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationchanged Asp371 to AsnPromoterT7 promoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK708
Plasmid#71692PurposeProduces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp400>Phe mutant (AarAH6-W400F)DepositorInsertcitrate synthase
UseTagsHis6ExpressionBacterialMutationchanged Trp400 to PhePromoterT7 promoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK521
Plasmid#72440PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)DepositorInserthypothetical protein
UseUnspecifiedTagsExpressionMutationsynthetic gene with ~40 silent substitutionsPromotern/aAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK536
Plasmid#72444PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)DepositorInserthypothetical protein
UseTagsExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF3_1
Plasmid#72363PurposeEncodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFPDepositorInsertZNF3 (ZNF3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF3_2
Plasmid#72364PurposeEncodes gRNA for 3' target of human ZNF3 along with Cas9 with 2A GFPDepositorInsertZNF3 (ZNF3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_PBX2_1
Plasmid#72357PurposeEncodes gRNA for 3' target of human PBX2 along with Cas9 with 2A GFPDepositorInsertPBX2 (PBX2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_PBX2_2
Plasmid#72358PurposeEncodes gRNA for 3' target of human PBX2 along with Cas9 with 2A GFPDepositorInsertPBX2 (PBX2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK539
Plasmid#72515PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)DepositorInsertformylglycinamidine ribonucleotide synthetase, large subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK555
Plasmid#72521PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)DepositorInsertformylglycinamidine ribonucleotide synthetase, glutaminase subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK577
Plasmid#72528PurposeEncodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)DepositorInsertformylglycinamidine ribonucleotide synthetase, small subunit
UseGene synthesisTagsExpressionMutationPromotern/aAvailable sinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
5CBD
Plasmid#69970PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPVX_084705:MAC04G-C11:C228845
UseBaculovirus expressionTagsHis and TEV cleavage siteExpressionMutationPromoterpolyhedrinAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
5C4Q
Plasmid#69963PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLdBPK_363130.1:MAC055-F03:C235029
UseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
psicheck_200_300_CA_repeat
Plasmid#65880PurposeLuciferase assay vector, assessing regulation of 200-300 nucleotide CA repeatsDepositorInsertCA repeats 200-300 nucleotides in length
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
psicheck_300_400_CA_repeat
Plasmid#65879PurposeLuciferase assay vector, assessing regulation of 300-400 nucleotide CA repeatsDepositorInsertCA repeats 300-400 nucleotides in length
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only