We narrowed to 21,834 results for: LEA

1. DS-ST1casN-

   Plasmid

   #48659

   Purpose

   Bacterial nuclease-null ST1 Cas9 expression

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9, nuclease-null
   ST1 tracrRNA
   

   Use

   CRISPR

   Mutation

   D to A, DH to AA, N to A

   Promoter

   proC

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. V-Kras

   Plasmid

   #27806

   Depositor

   Steven Vogel

   Article

   Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

   Insert

   Venus kras
   

   Expression

   Mammalian

   Available Since

   June 3, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pT7goldRNA

   Plasmid

   #47930

   Purpose

   In vitro transcription of golden gRNA

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   golden gRNA (slc24a5 Zebrafish)
   

   Use

   CRISPR

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSYC-181

   Plasmid

   #31566

   Depositor

   Seok-Yong Choi

   Article

   Kim et al PLoS One. 2011 . 6(4):e18556.

   Insert

   pCS4+-P2A
   

   Available Since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. DS-TDcasN-

   Plasmid

   #48660

   Purpose

   Bacterial nuclease-null TD Cas9 expression

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9, nuclease-null
   TD tracrRNA
   

   Use

   CRISPR

   Mutation

   D to A, DH to AA, N to A

   Promoter

   proC and tracrRNA promoter

   Available Since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUPER retro puro macroH2A1 shRNA

   Plasmid

   #30517

   Depositor

   John Gurdon

   Article

   Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

   Insert

   mH2A1 shRNA
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Map7emtb

   Plasmid

   #46078

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7emtb
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP8A-ccdB

   Plasmid

   #83883

   Purpose

   Destination vector for integration at HIS3, expression from the ADH1 promoter

   Depositor

   Jennifer Nemhauser

   Article

   Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. RHD_exon1_TALEN_R

   Plasmid

   #62240

   Purpose

   expression of TALEN targeting human RHD exon1

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

   Insert

   human RHD_exon1_TALEN_Right
   

   Use

   TALEN

   Tags

   HA tag, NLS

   Promoter

   CMV

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. RHD_exon1_TALEN_L

    Plasmid

    #62239

    Purpose

    expression of TALEN targeting human RHD exon1

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

    Insert

    human RHD_exon1_TALEN_Left
    

    Use

    TALEN

    Tags

    HA tag, NLS

    Promoter

    CMV

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUPER retro puro macroH2A2 shRNA

    Plasmid

    #30518

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    mH2A2 shRNA
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCYB-TnsA

    Plasmid

    #17356

    Depositor

    Nancy Craig

    Article

    Hickman et al Mol Cell. 2000 Jun . 5(6):1025-34.

    Insert

    TnsA (tnsA E. coli transposon Tn7)
    

    Tags

    Intein-Chitin binding domain

    Expression

    Bacterial

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pEC-K-CBP_CjeCas9

    Plasmid

    #83043

    Purpose

    Expresses Campylobacter jejuni Cas9 (Cje Cas9).

    Depositor

    Jennifer Doudna

    Article

    Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

    Insert

    CjeCas9
    

    Expression

    Bacterial

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pSYC-102

    Plasmid

    #74790

    Purpose

    pCS4+-NLS-EGFP-T2A-mCherry-CAAX

    Depositor

    Seok-Yong Choi

    Article

    Kim et al PLoS One. 2011 . 6(4):e18556.

    Insert

    NLS-EGFP-T2A-mCherry-CAAX
    

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-Hisx6-Sirt6-H133Y

    Plasmid

    #20279

    Depositor

    Leonard Guarente

    Article

    Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

    Insert

    Sirt6 (Sirt6 Mouse)
    

    Tags

    Hisx6

    Expression

    Bacterial

    Mutation

    Histidine 133 to Tyrosine

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pST50Trc4-STRNDHFR

    Plasmid

    #63996

    Purpose

    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    Strep II (STR); TEV cleavable

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3XFlag-YedK

    Plasmid

    #84567

    Purpose

    Expresses YedK in mammalian cells

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    YedK
    

    Tags

    3xFlag and C-Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST50Trc3-STRNDHFR

    Plasmid

    #63978

    Purpose

    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Tags

    Strep II (STR); TEV cleavable

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-HIS-TagGFP2-TEV-GGG-ELP(40nm)-Cys

    Plasmid

    #90474

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal TagGFP2 and TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Insert

    TagGFP2-TEV-GGG-ELP(40nm)-Cys
    

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL2-ptc-lu

    Plasmid

    #37678

    Depositor

    Philip Beachy

    Article

    Chen et al Cell. 1999 Aug 6;98(3):305-16.

    Insert

    ptc promoter (−758 to +130)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Promoter

    ptc

    Available Since

    Nov. 12, 2012

    Availability

    Academic Institutions and Nonprofits only