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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,992 results for: Met;

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Showing: 4841 - 4880 of 18992 results

  2. pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

    Plasmid
    #187844
    Purpose
    Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPe…
    Expression
    Mammalian
    Mutation
    meGFPe: asparagine 198 to aspartic acid and tyros…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187844-plasmid-map-sequence-id-379371

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  3. pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

    Plasmid
    #192785
    Purpose
    Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPe
    Expression
    Mammalian
    Mutation
    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192785-plasmid-map-sequence-id-379661

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  4. GEST-COMP-blac-flag-his

    Plasmid
    #111015
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    gamete egress and sporozoite traversal protein (GEST) (PF3D7_1449000 )
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111015-plasmid-map-sequence-id-224578

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  5. pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

    Plasmid
    #192704
    Purpose
    Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPm…
    Expression
    Mammalian
    Mutation
    meGFPm: gluatmic acid 142 to asparagine and histi…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192704-plasmid-map-sequence-id-379659

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  6. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid
    #192784
    Purpose
    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192784-plasmid-map-sequence-id-379372

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  28. pMuSIC pool v1.0

    Pooled Library
    #227193
    Purpose
    Two-way barcodes from 18 fluorescent proteins for mammalian expression. Can be used for clone tracing, or for pairing with perturbations for single cell resolution in spectral detection measurements.
    Depositor
    Marc Birtwistle
    Article
    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1
    Expression
    Mammalian
    Available Since
    Dec. 18, 2024
    Availability
    Academic Institutions and Nonprofits only

  30. G protein alpha-s-YFP

    Plasmid
    #55781
    Purpose
    This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    alpha-s-EE YFP (Gnas Rat)
    Tags
    EYFP flanked by SGGGS on each side was inserted i…
    Expression
    Mammalian
    Mutation
    Met was substituted for Gln69 in EYFP (Clontech).…
    Promoter
    CMV
    Available Since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    55781-plasmid-map-sequence-id-119279

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  31. pSicoR Dnmt1

    Plasmid
    #12166
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF Dnmt1 shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Insert
    Dnmt1 shRNA (Dnmt1 Mouse)
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Mutation
    EGFP is expressed from this plasmid as a marker, …
    Available Since
    June 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  32. EcoFlex MoClo Extension, Volume I

    Plasmid Kit
    #1000000253
    Purpose
    Use the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.
    Depositor
    Mikhail Shapiro
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only

  35. pGLU_113

    Plasmid
    #225794
    Purpose
    RS421 library variant 13453
    Depositor
    Nili Ostrov
    Insert
    13453_[Tn5]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225794-plasmid-map-sequence-id-447016

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  36. pGLU_95

    Plasmid
    #225809
    Purpose
    RS421 library variant 13453
    Depositor
    Nili Ostrov
    Insert
    13453_[Himar]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225809-plasmid-map-sequence-id-446989

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  37. pGLU_77

    Plasmid
    #225779
    Purpose
    RS421 library variant 13453
    Depositor
    Nili Ostrov
    Insert
    13453_[abr]
    Use
    Synthetic Biology
    Mutation
    N/A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225779-plasmid-map-sequence-id-446978

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  38. FRP935

    Plasmid
    #159302
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159302-plasmid-map-sequence-id-311152

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  39. MT1K

    Plasmid
    #105707
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 1k
    Depositor
    Artur Krężel
    Insert
    Metallothionein 1k
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Available Since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105707-plasmid-map-sequence-id-203327

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  40. pIAneo

    Plasmid
    #52118
    Purpose
    a bicistronic IRES-mAmetrine 1.1 containing vector for mammalian expression (CMV promoter)
    Depositor
    Dario Vignali
    Type
    Empty backbone
    Tags
    IRES-mAmetrine 1.1
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52118-plasmid-map-sequence-id-80504

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Showing: 4841 - 4880 of 18992 results