We narrowed to 10,093 results for: codon optimized

1. pBHM2642

   Plasmid

   #229526

   Purpose

   Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Tags

   t2a-mNeonGreen-mIAA7_opt

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJJR81

   Plasmid

   #75029

   Purpose

   tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Insert

   tagBFP2^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized tagBFP2

   Expression

   Worm

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-mNeonGreen

   Plasmid

   #124101

   Purpose

   Expression of the fluorescent protein mNeonGreen in mammalian cells

   Depositor

   Pantelis Tsoulfas

   Insert

   mNeonGreen
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Codon usage was optimized for Homo Sapiens

   Promoter

   CAG

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-L1PA13ACHmneo

   Plasmid

   #69613

   Purpose

   Expresses a neomycin tagged codon optimized L1PA13A

   Depositor

   Astrid Roy-Engel

   Insert

   L1PA13A
   

   Tags

   mneo retrotransposition cassette

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBHM2643

   Plasmid

   #229527

   Purpose

   Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

   Depositor

   Hiten Madhani

   Insert

   OsTIR1
   

   Tags

   t2a-mNeonGreen-mAID_opt

   Expression

   Bacterial and Yeast

   Mutation

   F74G

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103 STXBP4 WW domain

   Plasmid

   #104265

   Purpose

   Bacterial expression of WW domain from STXBP4

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   STXBP4 WW domain (STXBP4 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103 PIN1L WW domain

   Plasmid

   #104269

   Purpose

   Bacterial expression of WW domain from PIN1L

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   PIN1L WW domain (PIN1P1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTPGI_dCas9_VP64

   Plasmid

   #49013

   Purpose

   encodes yeast-optimized dCas9_VP64 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Yeast

   Promoter

   pTPGI (galactose+aTc inducible)

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_dCas9_VP64

   Plasmid

   #49015

   Purpose

   encodes human-optimized dCas9_VP64 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   dCas9_VP64 (human-codon-optimized)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGC4780

    Plasmid

    #113408

    Purpose

    Translation reporter with inducible translation coupling (high native GFP expression)

    Depositor

    Guillaume Cambray, Adam Arkin

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Insert

    158_33121123_1
    

    Use

    Synthetic Biology

    Tags

    fused to codon deptimized sfGFP with Flexible lin…

    Expression

    Bacterial

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGC4785

    Plasmid

    #113409

    Purpose

    Translation reporter with inducible translation coupling (low native GFP expression, high under coupling)

    Depositor

    Guillaume Cambray, Adam Arkin

    Article

    Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

    Insert

    359_31121113_2
    

    Use

    Synthetic Biology

    Tags

    fused to codon deptimized sfGFP with Flexible lin…

    Expression

    Bacterial

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Keasling-2337

    Plasmid

    #100168

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    lacIq-Ptrc-ADS
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

    Plasmid

    #163030

    Purpose

    Expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 pA, and myc NLS

    Expression

    Mammalian

    Promoter

    Alb-AAT

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBM019

    Plasmid

    #128179

    Purpose

    Construct for generating stable Cas9-expressing Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Inserts

    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Promoter

    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. (364) SB KRAB-SadCas9

    Plasmid

    #163022

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Promoter

    TET-On

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Flag-dSpCas9

    Plasmid

    #92113

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Keasling-2187

    Plasmid

    #100167

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    MevT and MBIS operon
    

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330-HA-dSpCas9

    Plasmid

    #92112

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9
    

    Use

    CRISPR

    Tags

    HA tag and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. HeFm2SpCas9

    Plasmid

    #92111

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    “Highly enhanced Fidelity” mut2 SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. (517) SB KRAB-SadCas9 U6-gStop

    Plasmid

    #163023

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Promoter

    Tet-On

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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