We narrowed to 5,091 results for: codon optimized

1. pMB34

   Plasmid

   #122245

   Purpose

   Lentivirus delivery for stable expression of LbCas12a-VPR

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Insert

   mammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator
   

   Use

   Lentiviral

   Tags

   NLS and VPR activator

   Expression

   Mutation

   Promoter

   EFs

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJV53-Cas12a

   Plasmid

   #158706

   Purpose

   CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   FnCpf1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetO

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSG5-ERRbeta2

   Plasmid

   #52186

   Purpose

   Expresses human ERRbeta2 splice variant in mammalian cells

   Depositor

   Rebecca Riggins

   Article

   Heckler et al Cell Cycle. 2014 Dec 12:0.

   Insert

   Estrogen-related receptor beta (ESRRB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild type sequence; codon optimized to exclude Ec…

   Promoter

   SV40

   Available since

   April 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-hsyn-FLPo-T2A-GFP

   Plasmid

   #161766

   Purpose

   express FLPo and GFP under the control of human synapsin promoter

   Depositor

   Baoji Xu

   Article

   An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

   Inserts

   FLPo
   T2AEGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRK1035

   Plasmid

   #8832

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

   Insert

   TVMV protease
   

   Use

   Tags

   His and MBP (cleaved)

   Expression

   Bacterial

   Mutation

   synthetic gene with codons optimized for E. coli

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

   Plasmid

   #160045

   Purpose

   For expressing the active auxin pump PIN2

   Depositor

   Michael Elowitz

   Article

   Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

   Insert

   PIN2
   

   Use

   Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   pEF1s

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RNF146-c005

   Plasmid

   #173091

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   RNF146 (RNF146 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   R99-L183, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Marathon-RT for Split-PE (pEH59)

   Plasmid

   #190108

   Purpose

   Expresses Marathon-RT (human codon optimization)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Use

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pALPS_3'LTR GFP@gag start SFFV tat-P2A-rev

   Plasmid

   #101340

   Purpose

   LTR drives GFP and internal SFFV promoter drives codon optimized tat and rev.

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   GFP, tat, rev
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2+_HA-mAID-nanobody (Kless)

    Plasmid

    #117718

    Purpose

    mRNA synthesis of lysine-less HA-mAID-nanobdy for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mAID-nanobody
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    codon-optimized and lysine-less mAID for murine e…

    Promoter

    SP6

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-FNLSHiFiNG-P2A-Puro

    Plasmid

    #136903

    Purpose

    Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFiNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

    Promoter

    EF1s

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSET-dCas9-actII-4-NT-S1

    Plasmid

    #110185

    Purpose

    Repression of gene expression in Streptomyces by CRISPRi

    Depositor

    Yinhua Lu

    Article

    Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

    Inserts

    dCas9
    sgRNA
    

    Use

    CRISPR; I

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    J23119 and ermE*p

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIntegrase_2

    Plasmid

    #60574

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. P823_eSpCas9(1.1)-RecA

    Plasmid

    #87264

    Purpose

    Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 3xFLAG-dCas9/pMXs-neo

    Plasmid

    #51260

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FLAG-TKGAPDH in pLJM60

    Plasmid

    #154217

    Purpose

    Lentiviral vector for stable expression of FLAG-TK-GAPDH

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    GAPDH
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    cDNA has been codon optimized for human cell expr…

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-nucGFP11-3xControlgRNA

    Plasmid

    #224568

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    GFP11-H2B
    

    Use

    CRISPR

    Tags

    Histone H2B (nuclear localization)

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEYFP-N1-eKR2

    Plasmid

    #115337

    Purpose

    Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

    Depositor

    Peter Hegemann

    Article

    Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

    Insert

    eKR2
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3xFlag-Dam

    Plasmid

    #121909

    Purpose

    Expresses E. coli Dam methyltransferase containing an N-ternimal 3xFlag tag

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    Dam human codon optimized (dam E. coli)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSW002-PpsbA_mTurquoise2

    Plasmid

    #108237

    Purpose

    Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    PsbA promoter - mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge