We narrowed to 20,504 results for: ACE

1. pBP-TL11

   Plasmid

   #72995

   Purpose

   RBS_L4

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   L4 RBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pQE-WT-CTGA-R13-AKSIRV

   Plasmid

   #63627

   Purpose

   CTGA-R13-AKSIRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   CTGA-R13-AKSIRV RNAP
   

   Use

   Tags

   n/a

   Expression

   Mutation

   V725A, Q744K, L747I, N748S, L749I, R756T, Q758K, …

   Promoter

   T5/Lac

   Available Since

   June 3, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. BASIC_7_J23101-RBS34-mCherry-B0015

   Plasmid

   #68141

   Purpose

   storage vector for BASIC part: RFP expression cassette

   Depositor

   Geoffrey Baldwin

   Article

   Storch et al ACS Synth Biol. 2015 Apr 16.

   Insert

   J23101-RBS34-mcherry-B0015
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-Split-Broccoli-Toehold-Switch

   Plasmid

   #87316

   Purpose

   Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P70a

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE-WT-N4-R5-RNRV

   Plasmid

   #63630

   Purpose

   N4-R5-RNRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   N4-R5-RNRV RNAP
   

   Use

   Tags

   Expression

   Mutation

   L747I, N748D, L749C, M750V, F751I, Q754T, F755R, …

   Promoter

   T5/Lac

   Available Since

   April 15, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSecEffector

   Plasmid

   #172891

   Purpose

   Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   Bxb1
   TP901
   TEV Display cassette
   ProD promoter between anti aligned Bxb1 sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ProD, nTETO, and pBAD /AraC

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH75111

   Plasmid

   #50327

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, beta-glucuronidase (E. coli) with two introns,
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFTK046

   Plasmid

   #171318

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   DsRed.T1 fluorescent reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Promoter

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM705

   Plasmid

   #68892

   Purpose

   IPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQLinkHD_EBFP2

    Plasmid

    #118853

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    EBFP2
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    EBFP1.2-I128V/V150I/ D155V/V224R

    Promoter

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICSL30005

    Plasmid

    #50299

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    N terminal FLAG tag (3x FLAG octapeptide)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL50006

    Plasmid

    #50319

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, luciferase (Photinus pyralis)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Golgi-DKAR

    Plasmid

    #49790

    Purpose

    FRET-based, Golgi-targeted kinase activity reporter for PKD

    Depositor

    Alexandra Newton

    Article

    Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

    Insert

    D kinase activity reporter
    

    Use

    Tags

    CFP, YFP, and eNOS(1-33)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pZA2[PTAN:GFP]

    Plasmid

    #60779

    Purpose

    Contains PTAN driving expression of GFP

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Insert

    yemGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NAT-TET-OFF -LEU- 4xFLAG

    Plasmid

    #182527

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    7 Copies of TetO Box
    

    Use

    Tags

    4XFLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICH45152

    Plasmid

    #50263

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter, cab1 (AT1g29930, A. thaliana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICH71421

    Plasmid

    #51834

    Purpose

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    plant 3'UTRpolyadenylation signal/terminator, Tom H4
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBLlSh2

    Plasmid

    #170981

    Purpose

    Expression of ShCAST under control of lacI Plac promoter/repressor pair.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Inserts

    lacI
    ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLlac01

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICH42211

    Plasmid

    #50255

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter nos (A. tumefaciens)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHelper_O

    Plasmid

    #173783

    Purpose

    The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    186 Integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    E59G - please see depositor comments

    Promoter

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge