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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,492 results for: MEA;

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Showing: 2441 - 2460 of 2492 results

  1. pIS1 mEPAS1

    Plasmid
    #60793
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains EPAS1 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    EPAS1 3'UTR and mutated miR-155 binding site (EPAS1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available Since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    60793-plasmid-map-sequence-id-112560

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  2. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available Since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin

    Plasmid
    #231549
    Purpose
    Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    ACTB (ACTB Human)
    Tags
    msfGFP (intramolecular)
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231549-plasmid-map-sequence-id-459702

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  4. pCMVtrunc msfGFPΔC10-beta-actin

    Plasmid
    #231548
    Purpose
    Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    ACTB (ACTB Human)
    Tags
    msfGFP
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231548-plasmid-map-sequence-id-459700

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  5. Desmoplakin II donor only control (F40-based tension sensor)

    Plasmid
    #118717
    Purpose
    The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118717-plasmid-map-sequence-id-230455

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  6. pCMVtrunc msfGFPΔC10-Utr28-222

    Plasmid
    #231558
    Purpose
    Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Article
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Utrophin calponin homology domain (UTRN Human)
    Tags
    msfGFP
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231558-plasmid-map-sequence-id-459698

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  8. AIMTOR T757

    Plasmid
    #140828
    Purpose
    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells
    Depositor
    Anne Bonnieu
    Article
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Insert
    AIMTOR T757 (ULK1 Human)
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140828-plasmid-map-sequence-id-305141

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  9. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Article
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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  20. PRESTO-Tango Kit

    Plasmid Kit
    #1000000068
    Purpose
    Used to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.
    Depositor
    Bryan Roth
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2441 - 2460 of 2492 results