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Plasmid#91695PurposeDicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtrDepositorInsertSpCas9
TagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9PromoterPcUbiAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPRv2FE-ABE8e-SpRY
Plasmid#213008PurposeA lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning siteDepositorInsertABE8e-SpRY-D10A
UseCRISPR and LentiviralExpressionMammalianMutationD10A nickase variant of SpRYPromoterEf-1aAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_Ado1.0med
Plasmid#153286PurposeExpress the medium-affinity adenosine sensor GRAB_Ado1.0med in neuronsDepositorHas serviceAAV2InsertAdenosine sensor GRAB_Ado1.0med (medium affinity)
UseAAVPromoterhSynAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-MyosinIIB-C-18
Plasmid#54192PurposeLocalization: Cytoskeleton, Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMyosinIIB (MYH10 Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
mEmerald-Inversin-C-18
Plasmid#54131PurposeLocalization: Cilium, Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertInversin (INVS Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
PA-TagRFP-pBad
Plasmid#62793PurposeLocalization: Protein Expression Vector , Excitation:N/A, Emission:N/ADepositorTypeEmpty backboneTags6xHis and TagRFPExpressionBacterialMutationnoneAvailable sinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCasTet-λ
Plasmid#128318PurposeConstitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.DepositorInsertTetR and pTetR/TetO
UseCRISPR and Synthetic BiologyExpressionBacterialMutationConstitutive expression of cas9 and anhydro-tetra…Promotertet promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
STING-V2
Plasmid#124264PurposeC-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartmentsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTMEM173 (STING1 Human)
TagsVenus2ExpressionMammalianAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SypHer2-DAAO-NES
Plasmid#119184PurposeFusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.DepositorInsertSypHer2-DAAO-NES
UseAAVTagsNESExpressionMammalianPromoterCMVAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHJ12: CadC-VHH-Caffeine (No linker)
Plasmid#108244PurposeMembrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expressionDepositorInsertMembrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
UseSynthetic BiologyPromoterpLacO1Available sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY2low-mCherry
Plasmid#104065PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRY2low-mCherry
ExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSHS207 - Bacterial expression plasmid for WT SpCas9
Plasmid#101199PurposeBacterial expression plasmid for WT SpCas9DepositorInsertSpCas9
Tags10x His, MBP, and TEV siteExpressionBacterialPromoterT7Available sinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEos3.2-TOMM20-N-10
Plasmid#57483PurposeLocalization: Mitochondrial Membrane, Excitation: 507 / 572, Emission: 516 / 580DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTOMM20 (TOMM20 Human)
TagsmEos3.2ExpressionMammalianPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pcDNA5/FRT/TO-GAlpha11-RLuc8
Plasmid#140983PurposeEncodes a G alpha subunit (GNA11) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlpha11-RLuc8 (GNA11 Human)
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPACE-NG (pRZ5150)
Plasmid#140244PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSPACE_NG
ExpressionMammalianMutationV82G in TadA*, NG mutations in SpCas9, D10A in Sp…PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CLXSN 5myc-CDC6-WT
Plasmid#109332Purposeexpress tagged Cdc6 WTDepositorAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
(R42A) Cyclin B1-GFP (62)
Plasmid#61849Purposemammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructableDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcyclin B1 (CCNB1 Human)
TagsGFP and MycExpressionMammalianMutationV5A R42APromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-FLEX-FLIM-AKAR
Plasmid#60445PurposeExpresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backboneDepositorInsertFLIM-AKAR
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EBFP2-Nucleus-7
Plasmid#55249PurposeLocalization: Nuclear Localization, Excitation: 383, Emission: 448DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSV40 T-Antigen Nuclear Localization Signal (NLS)
TagsEBFP2-NLSExpressionMammalianPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
Clover2-C1
Plasmid#54711PurposeLocalization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.DepositorTypeEmpty backboneTagsClover2ExpressionMammalianAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by