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pKJ0463

Plasmid

#228715

Purpose

Bacterial expression of epT7 RNAP; for protein production and purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Sumo_epT7

Expression

Bacterial

Mutation

T3M/G47A/E643G

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCBL101gg

Plasmid

#199735

Purpose

Golden Gate recipient vector for transgene overexpression

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

sacB

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJC53.2

Plasmid

#26536

Purpose

T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Depositor

Phillip Newmark

Article

Collins et al PLoS Biol. 2010 . 8(10):e1000509.

Type

Empty backbone

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pET15b-ALFA-TwinStrep

Plasmid

#240252

Purpose

IPTG-inducible plasmid with thermostable GFP (TGP) and Twin-Strep tags

Depositor

Olga Boudker, Krishna Reddy

Article

Reddy et al Nat Struct Mol Biol (2025).

Type

Empty backbone

Tags

10x HIS, ALFA, Thrombin, and Twin-Strep

Expression

Bacterial

Promoter

T7

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC1_N-mEGFP

Plasmid

#231271

Purpose

For stably expressing fusion proteins with N terminally tagged mEGFP in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC1_C-mCitrine

Plasmid

#231281

Purpose

For stably expressing fusion proteins with C terminally tagged mCitrine in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mCitrine

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAVEC1_C-TurboID

Plasmid

#231285

Purpose

For stably expressing fusion proteins with C terminal TurboID for proximity labeling in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

TurboID

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pUA66 PrpsL GFP

Plasmid

#105606

Purpose

Expresses GFP under PrpsL

Depositor

Pamela Silver

Article

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Expression

Bacterial

Promoter

PrpsL

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+ centrin-RFP

Plasmid

#26753

Depositor

Christopher Kintner

Article

Park et al Nat Genet. 2008 Jul . 40(7):871-9.

Insert

Centrin 4 (cetn4 Frog)

Use

Reporter for basal body

Tags

monomeric RFP

Available Since

Feb. 8, 2011

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-mCherry

Plasmid

#87664

Purpose

retroviral vector encoding cre dependent expression of membrane mCherry

Depositor

Oscar Marin

Article

Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

Insert

Mem-mCherry

Use

Retroviral

Promoter

CAG

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAVEC1_C-mEGFP

Plasmid

#231279

Purpose

For stably expressing fusion proteins with C terminally tagged mEGFP in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mEGFP

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-NLS-HA-B3

Plasmid

#51270

Purpose

Expression of the site-specific recombinase B3 (mouse codon-optimized)

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-B3

Expression

Mammalian

Promoter

CAG

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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XW12

Plasmid

#65834

Purpose

Pquas-Δpes10-GFP-3'UTR-unc-54

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

5XQUAS-delta-pes-10

Tags

GFP

Expression

Worm

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pKJ0377

Plasmid

#228712

Purpose

Mammalian expression of human codon-optimized enhanced Bxb1 (epBxb1 mutant T166R)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Bxb1_T166R

Expression

Mammalian

Mutation

T166R

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1 Flag BAF155 b

Plasmid

#1954

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BAF155 (SMARCC1 Human)

Tags

flag

Expression

Insect

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pPolII-Luc

Plasmid

#17223

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

PolII promoter

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
ZE26 Stbm-N-6myc-pCS2+

Plasmid

#17068

Depositor

Randall Moon

Article

Park et al Nat Cell Biol. 2002 Jan;4(1):20-5.

Insert

Stbm (vangl2 Zebrafish)

Use

Zebrafish expression

Tags

Myc6

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pSB305-K3L-vac

Plasmid

#20053

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

K3L

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pBTK1056

Plasmid

#209546

Purpose

BTK Type 4 antibiotic resistance cassette for Golden Gate assembly

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv [Preprint]. 2023 Sep 20:2023.09.

Insert

Type 4 Kanamycin resistance cassette

Use

Synthetic Biology

Available Since

Jan. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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p8RwB

Plasmid

#48733

Purpose

dsRed plasmid

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

dsRed-Express

Expression

Mammalian

Promoter

EF1a

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-Histone H1

Plasmid

#45083

Depositor

Phillip Newmark

Article

Rouhana et al Development. 2014 Jul;141(13):2592-601.

Insert

Histone H1

Use

RNAi; In vitro transcription

Promoter

T7 sense, T3 antisense

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pJW1673

Plasmid

#112047

Purpose

CEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

SCT1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGKO1

Plasmid

#63611

Purpose

a binary Agrobacterium vector carrying the HSVtk gene as a negative selection marker against ectopic transformants.

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Binary fungal vector

Promoter

Lac Promoter

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBLggPro

Plasmid

#199736

Purpose

Golden Gate entry vector for promoter cloning

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Expression

Plant

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBLggCDS

Plasmid

#199737

Purpose

Golden Gate entry vector for gene of interest cloning

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBLggTerm

Plasmid

#199738

Purpose

Golden Gate entry vector for terminator cloning

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Type

Empty backbone

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB305-K3L-vac-HA

Plasmid

#20054

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

K3L

Tags

HA

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pLLU2G-Oct3

Plasmid

#21616

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Oct3 shRNA

Use

Lentiviral and RNAi

Available Since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
EF2451_pET21a(+)

Plasmid

#13840

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

PPAT

Tags

His

Expression

Bacterial

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+ tecktin-GFP

Plasmid

#26932

Depositor

Christopher Kintner

Article

Park et al Nat Genet. 2008 Jul . 40(7):871-9.

Insert

Tektin 2 (tekt2 Frog)

Tags

GFP

Expression

Mammalian

Mutation

I429A

Available Since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only
pVB004

Plasmid

#127188

Purpose

Tunable NOT gate without RiboJ insulator

Depositor

Thomas Gorochowski

Article

Bartoli et al Nat Commun. 2020 Apr 29;11(1):2095. doi:

Insert

Tunable NOT gate and Reporter without RiboJ Insulator

Expression

Bacterial

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1671

Plasmid

#112045

Purpose

CEN/ARS plasmid for GAL-inducible ORM1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ORM1

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW1675

Plasmid

#112049

Purpose

CEN/ARS plasmid for GAL-inducible TSC13-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

TSC13

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB819-PKR-gib

Plasmid

#20031

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pLLNeo-Oct3

Plasmid

#21618

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Oct3 shRNA

Use

Lentiviral and RNAi

Available Since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB819-PKR-titi

Plasmid

#20035

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pSB305-K3L-var

Plasmid

#20055

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

K3L

Expression

Yeast

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-ora-L394F

Plasmid

#20052

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Mutation

L394F

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pAP697e

Plasmid

#253944

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Article

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Linker::T2A::Linker::mScarlet::Linker+CAAX

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pART7

Plasmid

#247985

Purpose

Cloning vector to transiently express an inserted gene in plant cells under the control of the 35S CaMV promoter.

Depositor

Andrew Gleave, Christoph Plieth

Article

Gleave et al Plant Mol Biol. 1992 Dec;20(6):1203-7.

Type

Empty backbone

Expression

Plant

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC0_N-TurboID

Plasmid

#231261

Purpose

For expressing fusion proteins with N terminal TurboID for proximity labeling in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

TurboID

Expression

Plant

Mutation

N/A

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC1_N-TurboID

Plasmid

#231277

Purpose

For stably expressing fusion proteins with N terminal TurboID for proximity labeling in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

TurboID

Expression

Plant

Mutation

N/A

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC0_N-Renilla

Plasmid

#231259

Purpose

For expressing fusion proteins with N terminally tagged Renilla luciferase in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Luciferase

Tags

Renilla

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC0_C-Renilla

Plasmid

#231267

Purpose

For expressing fusion proteins with C terminally tagged Renilla luciferase in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Luciferase

Tags

Renilla

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC0_C-TurboID

Plasmid

#231269

Purpose

For expressing fusion proteins with C terminal TurboID for proximity labeling in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

TurboID

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC1_N-mTurquoise2

Plasmid

#231270

Purpose

For stably expressing fusion proteins with N terminally tagged mTurquoise2 in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mTurquoise2

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC1_N-mNeonGreen

Plasmid

#231272

Purpose

For stably expressing fusion proteins with N terminally tagged mNeonGreen in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mNeonGreen

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC1_N-mCitrine

Plasmid

#231273

Purpose

For stably expressing fusion proteins with N terminally tagged mCitrine in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mCitrine

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC1_N-Renilla

Plasmid

#231275

Purpose

For stably expressing fusion proteins with N terminally tagged Renilla luciferase in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Use

Luciferase

Tags

Renilla

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAVEC1_N-StrepIII

Plasmid

#231276

Purpose

For stably expressing fusion proteins with N terminal StrepIII tags in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

StrepIII

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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