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pEGFP-NMHC-IIA-3xA

Plasmid

#101041

Purpose

Expresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.

Depositor

Tom Egelhoff

Article

Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311

Insert

Non-muscle myosin IIA heavy chain (MYH9 Human)

Tags

GFP

Expression

Mammalian

Mutation

S1915A, S1916A, S1943A

Promoter

CMV

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLV Cbx3-V5

Plasmid

#175104

Purpose

Lentiviral expression of mouse Cbx3-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Cbx3 (Cbx3 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCINeo-mCHMP5

Plasmid

#11771

Depositor

Sankar Ghosh

Article

Shim et al J Cell Biol. 2006 Mar 27. 172(7):1045-56

Insert

chromatin modifying protein 5 (Chmp5 Mouse)

Expression

Mammalian

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
PA-mCherry-miniSOG-Zyxin-6

Plasmid

#57802

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Zyxin (ZYX Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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FusionRed-CD36-C-10

Plasmid

#56104

Purpose

Localization: Membrane, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

CD36 (CD36 Human)

Tags

FusionRed

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCherry-miniSOG-Actin-7

Plasmid

#55085

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
miniSOG-Alpha-V-Integrin-25

Plasmid

#57763

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Alpha-V Integrin (ITGAV Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pFRT-DHX36iso2E335A

Plasmid

#159588

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

E335A

Promoter

CMV

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114104

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

sbGLuc-VChR1-EYFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Vimentin-7

Plasmid

#57800

Purpose

Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vimentin (VIM Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-C

Plasmid

#128417

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

INTS3 C-terminal region (514-1042 aa)

Promoter

Tac promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Golgi-7

Plasmid

#57791

Purpose

Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Golgi (B4GALT1 Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pRC/RSV-rat Agt

Plasmid

#122104

Purpose

Expression of angiotensinogen

Depositor

John Chan

Article

Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

Insert

angiotensinogen (Agt Rat)

Expression

Mammalian

Promoter

RSV promoter

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-EB3-7

Plasmid

#55089

Purpose

Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

EB3 (MAPRE3 Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-VE-Cadherin-N-10

Plasmid

#57799

Purpose

Localization: Tight Junctions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

VE-Cadherin (CDH5 Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Clathrin-15

Plasmid

#55088

Purpose

Localization: Clathrin Vesicles, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Clathrin (CLTB Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
miniSOG-EB3-7

Plasmid

#57765

Purpose

Localization: MT End Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

EB3 (MAPRE3 Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Frt-V5-hspB3

Plasmid

#63104

Purpose

Expression of HSPB3 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb3 (HSPB3 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-CDC42-C-10

Plasmid

#55087

Purpose

Localization: Membrane, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

CDC42 (CDC42 Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
miniSOG-Keratin-17

Plasmid

#57770

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Keratin (KRT18 Human)

Tags

miniSOG

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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